Effects of N-Acetyl cysteine on gene expression of Protein kinase C-α and Ankyrin repeat domain-containing protein 1 in the cardiac tissue of rats exposed to lead

Keywords

Antioxidants, cardiac tissue, lead, N-Acetyl cysteine, PKC-α and ANKRD1 genes

How to Cite

Gholami, M., Ghahraman, P., Beigi Harchegani, A., Mohsenifar, Z., & Parvizi, M. R. (2022). Effects of N-Acetyl cysteine on gene expression of Protein kinase C-α and Ankyrin repeat domain-containing protein 1 in the cardiac tissue of rats exposed to lead . Iranian Red Crescent Medical Journal, 24(1). Retrieved from https://ircmj.com/index.php/IRCMJ/article/view/1584

Abstract

Objective: This study aimed to evaluate the effects of N-acetylcysteine (NAC) on the oxidative damage, inflammation and expression of PKC-α and ANKRD1 genes in the cardiac tissue of rats exposed to lead (Pb).

Methods: Rats were randomly divided into five groups including G1 (control), G2 (single dose of Pb), G3 (continuous dose of Pb), G4 (single dose of Pb + NAC), and G5 (continuous dose of Pb + NAC). Levels of malondialdehyde (MDA), total antioxidant capacity (TAC), IL-10 and TNF-α were measured. Expression of PKC-α and ANKRD1 genes was considered using RT-PCR.

Results: Continuous exposure to Pb caused a significant decrease in serum levels of TAC and IL-10, but increased MDA and TNF-α contents (p<0.001). Continuous dose of Pb also dramatically increased the expression of PKC-α and ANKRD1 genes in the cardiac tissue by 4.27-fold and 3.07-fold, respectively (p<0.001). NAC treatments not only improved morphological changes, oxidative stress and inflammatory biomarkers, but also compensated antioxidant capacity and expression of PKC-α and ANKRD1 genes in cardiac tissue.   

Conclusion: Pb exposure is strongly associated with cardiotoxicity through inducing oxidative stress, inflammation, and antioxidant depletion. NAC ameliorates Pb-induced cardiotoxicity by elevating antioxidants capacity, mitigating oxidative stress and down-regulating of PKC-α and ANKRD1 genes.

 

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