Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR

AUTHORS

F Khatami 1 , Mohammad Mehdi Heidari 2 , * , M Khatami 2

1 Department of Biology, Science School, Ferdowsi University, Mashhad, Iran.

2 Department of Biology, Science School, Yazd University, Yazd, Iran.

How to Cite: Khatami F, Heidari M M, Khatami M. Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR, Iran Red Crescent Med J. 2012 ; 14(9):e95966.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 14 (9); e95966
Published Online: September 30, 2012
Article Type: Brief Report
Received: July 03, 2019
Accepted: December 20, 2011

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Abstract

Background: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans.

Methods: We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7.

Result: PCR product of 336 bp was successfully amplified in a FLASH–PCR.

Conclusion: As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.

Keywords

coli O157:H7 Stx-1 gene Detection FLASH-PCR

© 2012, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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