Detection and Prevalence of Polyoma Virus BK among Iranian Kidney Transplant Patients by a Novel Nested-PCR

AUTHORS

M Shenagari 1 , M Ravanshad 2 , * , SY Hosseini 3 , R Ghanbari 1

1 Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2 Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, PO Box: 14115-331, [email protected], Tehran, Iran

3 Gastroenterohepatology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Fars, Iran

How to Cite: Shenagari M, Ravanshad M, Hosseini S, Ghanbari R. Detection and Prevalence of Polyoma Virus BK among Iranian Kidney Transplant Patients by a Novel Nested-PCR, Iran Red Crescent Med J. Online ahead of Print ; 12(6):631-635.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 12 (6); 631-635
Article Type: Research Article
Received: December 12, 2009
Accepted: April 12, 2010

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: BK virus is an increasingly recognized pathogen in transplant recipient patients associated with nephropathy and emerged as a cause of allograft failure linked to immunosuppressive regimens in renal transplant recipients. This study develops a sensitive PCR method to detect the viremia and viruria as well as the incidence of BK virus infection in renal transplant recipients.

 

Methods: A nested PCR method was developed and a total of 45 paired serum and urine samples from renal transplant recipient patients were collected and tested with the developed assay.

 

Results: The threshold of the developed detection assay was 10 copies/µl of BKV DNA in samples. Our results also indicated that about 40% of the urine and 26.7% of serum samples were positive for BKV in renal transplant patients in this study.

 

Conclusion: This Nested-PCR method was found a specific, sensitive and simple procedure for detection of viruria and viremia of BK virus in renal transplant recipients.

Keywords

BK virus Renal transplant recipients Prevalence Iran

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: