The effect of 0.02% Mitomycin-C drop in prevention of epithelial ingrowth in corneal stroma of rabbit as an animal experimental model

AUTHORS

A Katbab 1 , * , F Dabiri 2 , H Movaheddan Movaheddan 3 , R Salouti 2 , H Khoshniat 2 , HR Jahadi 2 , M Nejabat 2 , M Eghtedari 2

1 Poostchi Eye Research Center, Shiraz University of Medical Sciences, Zand Street, [email protected], Fars, Iran

2 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Fars, Iran

3 Shiraz, Iran

How to Cite: Katbab A, Dabiri F, Movaheddan H, Salouti R, Khoshniat H, et al. The effect of 0.02% Mitomycin-C drop in prevention of epithelial ingrowth in corneal stroma of rabbit as an animal experimental model, Iran Red Crescent Med J. Online ahead of Print ; 10(2):107-110.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 10 (2); 107-110
Article Type: Research Article
Received: May 12, 2007
Accepted: November 18, 2007

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Abstract

Background: Mitomycin–C (MM-C) is an alkylating anti-tumor antibiotic, which interrupts DNA replication and inhibits routine synthesis and cell mitosis. This study was undertaken to evaluate the effect of MM-C on the epithelial ingrowth which is a sight threatening complication after corneal surgeries.

 

Methods: This study was conducted on 50 corneas of 25 Dutch Albino rabbits. Lamellar keratectomy was performed and after the application of MM-C 0.02 % or balanced salt solution (control group), the epithelial cells were implanted on the stromal bed. After sixty days, light microscopic exam was performed on the excised corneas.

 

Results: Three eyes in the MM-C group and no eye in the control group showed epithelial cell growth under the corneal flap.

 

Conclusion: In this study, application of MM-C 0.02% drop at the time of operation had no preventive role in the corneal epithelial cell proliferation under rabbit’s corneal flap. So, implantation of the epithelial cells under the corneal flap is not the sole causative factor of epithelial ingrowth in the stroma of the rabbit’s cornea.

 

Keywords

Mitomycin C Epithelial cell Cornea Balanced salt solution

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