Thioacetamide-Induced Acute Hepatic Encephalopathy in Rat: Behavioral, Biochemical and Histological Changes


M Farjam 1 , S Dehdab 1 , F Abbassnia 1 , D Mehrabani 2 , * , N Tanideh 1 , S Pakbaz 1 , MH Imanieh 1

1 Department of Pharmacology, Shiraz University of Medical Sciences, Iran

2 Assistant Professor of Stem Cell Research and Transgenic Technology Research Center, Shiraz University of Medical Sciences, [email protected], Iran

How to Cite: Farjam M, Dehdab S, Abbassnia F, Mehrabani D, Tanideh N, et al. Thioacetamide-Induced Acute Hepatic Encephalopathy in Rat: Behavioral, Biochemical and Histological Changes, Iran Red Crescent Med J. Online ahead of Print ; 14(3):164-170.


Iranian Red Crescent Medical Journal: 14 (3); 164-170
Article Type: Research Article
Received: September 30, 2011
Accepted: January 10, 2012




Background: As a serious neuropsychiatric disease, hepatic encephalopathy (HE) is a clinical condition with several types regarding chronicity and clinical diversity that can develop as a complication of both acute and chronic liver failure. This study evaluates changes in thioacetamide (TAA)-induced acute hepatic encephalopathy (AHE) in rat as an animal model.


Methods: Both genders of C57BL6, BALB/C mice and Sprague Dawley rats; (10 animals in each group) were compared for induction of AHE to clarify which animal and gender were appropriate. The animals (10 male rats in each group) were categorized in 4 groups according to the dose of the TAA administered (200, 300 and 400 mg/kg of TAA at 24 h intervals for 4 days). A control group was treated with solvent of TAA which was water (5 ml/kg/day). The behavioral, biochemical markers of hepatic failure and histological aspects of thioacetamide (TAA) induced AHE and the correlation between the clinical severity and liver failure biomarkers were evaluated.


Results: Rat was shown to be an animal model of choice for AHE while the optimum dosage of TAA to induce AHE was 300 mg/kg/day at 24 h intervals for 4 days. The behavioral score was partially correlated with the rising of some biomarkers and pathological findings.  


Conclusion: Rat can be introduced as the animal of choice for AHE to study the pathophysiology, pharmacology and the survival rate of disease in liver transplant patients. 


Acute hepatic encephalopathy Thioacetamide Rat

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF