In Vitro Antioxidant Properties Evaluation of 10 Iranian Medicinal Plants by Different Methods

AUTHORS

Soheila Moein 1 , Mahmoodreza Moein 2 , * , Mohammad Javad Khoshnoud 3 , Tahereh Kalanteri 2

1 Department of Biochemistry, Faculty of Medicine and Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, IR Iran

2 Medicinal Plants Research Center and Department of Pharmacognosy, Faculty of Pharmacy, Shiraz University of Medical Sciences, [email protected], IR Iran

3 Pharmaceutical Sciences Research Center and Department of Toxicology and Pharmacology, Faculty of Pharmacy, Shiraz University of Medical Sciences, IR Iran

How to Cite: Moein S, Moein M, Khoshnoud M J, Kalanteri T. In Vitro Antioxidant Properties Evaluation of 10 Iranian Medicinal Plants by Different Methods, Iran Red Crescent Med J. Online ahead of Print ; 14(12):771-775. doi: 10.5812/ircmj.1408.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 14 (12); 771-775
Published Online: December 6, 2012
Article Type: Research Article
Received: July 4, 2011
Accepted: February 15, 2012
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Abstract

Background: There is an interest in finding new and safe antioxidants from natural sources such as medicinal plants.

Objectives: The aim of this study was to evaluate the antioxidant activity of ten Iranian medicinal plants extracts.

Materials and Methods: For antioxidant activity, the radical scavenging activity, reducing power and phenolic contents of ethanol plant extracts were determined. Gallic acid was used as standard reference with well-documented antioxidant activity.

Results: The highest antioxidant activity in terms of DPPH radical scavenging was found in Verbascum sinuatum L. Var (VS) with an IC50 equal to 263.52 5.981 ?g/ml and Rosa damascena Mill (RD) with and IC50 equal to 287.9 5.675 ?g/ml that are higher than gallic acid (IC50 = 25.32 5.593 ?g/ml). The highest antioxidant activity in terms of ferric reducing capacity was also found in Verbascum sinuatum L. Var extracts (in 85.08 8.66 ?g/ml concentration with absorbance 0.5). Also, this extract contains the highest phenolic compounds (8.53 0.11 mg/g).

Conclusion: In this study, Verbascum sinuatum L. Var contains the highest level of phenolic compounds may be contribute to higher free radical scavenging activity and reducing power in comparison to the other plant extracts. Therefore this plant is a good candidate as natural antioxidant.

Keywords

Phenolic Compounds Radical Scavenging Power

© 2012, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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