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The Use of pp150 and gp116 Synthetic Peptides in the Detection of CMV Antibodies

AUTHORS

Foroogh Nejatollahi 1 , * , I Alshami 2 , M Moazen 3 , N Farahbakhsh 4

1 Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Fars, Iran

2 Department of Medical Microbiology, University of Manchester, UK ,

3 Jahrom Payame Noor University, Jahrom, Fars, Iran

4 Jahrom Medical School, Jahrom, Iran ,

How to Cite: Nejatollahi F, Alshami I, Moazen M, Farahbakhsh N. The Use of pp150 and gp116 Synthetic Peptides in the Detection of CMV Antibodies, Iran Red Crescent Med J. 2010 ; 12(1):e95880.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 12 (1); e95880
Published Online: January 31, 2010
Article Type: Research Article
Received: July 01, 2019
Accepted: January 31, 2010

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Abstract

Background: Human cytomegalovirus (HCMV) infection may cause severe outcomes in transplant recipients and pregnant women. Diagnosis of CMV infections by using serological detection of CMV specific antibodies varies widely due to different antigen compositions in the diagnostic tests. The aim of this study wasto identify the reactive peptides of CMV for CMV-IgM and –IgG detection.

  Methods: The reactivity of peptides (peptide 1:  amino acids 595-614 of phosphoprotein 150 (pp150),  peptide2: amino acides 1024-1048 of pp150, peptide3: amino acids 798-805 of C-terminal part of glycoprotein B (gp55), peptide 4: amino acids 68-81 of N-terminal part of glycoprotein B (gp 116), and peptide 5: amino acids 29-48 of glycoprotein H)  as epitopes  was determined in ELISA, using renal transplant recipients’ sera (n=84) with high titers of CMV-IgM and healthy individuals’ sera (n=87) with high titers of CMV-IgG antibodies.

 Results: Amino acids 595-614 and 1024-1048 of pp150 had a high reactivity (83.3% and 88.0%) with renal transplant recipients' sera while the other peptides did not. The amino acids 68-81 of gp116 reacted with 81.6% of the healthy individuals’ sera but the other amino acids showed low reactivity with these sera.

 Conclusion: Amino acids 595-614 and 1024-1048 of pp150 and amino acids 68-81 of N-terminal part of gp 116 could be considered in recombinant protein construction for detection of CMV-IgM and -IgG antibodies.

Keywords

CMV IgM IgG Fusion protein Peptides pp150 gp116 gp H

© 2010, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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