Iranian Red Crescent Medical Journal

Published by: Kowsar

Distribution of Virulence Genes in Moraxella catarrhalis Isolated from Clinical Samples in the North of Iran

Mina Eghbali 1 , 2 , Majid Baserisalehi 3 , * and Masood Ghane 4
Authors Information
1 Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran
2 Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
3 Department of Microbiology, Kazeron Branch, Islamic Azad University, Kazeron, Iran
4 Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Article information
  • Iranian Red Crescent Medical Journal: 21 (1); e84125
  • Published Online: February 3, 2019
  • Article Type: Research Article
  • Received: September 9, 2018
  • Revised: December 22, 2018
  • Accepted: December 25, 2018
  • DOI: 10.5812/ircmj.61381

To Cite: Eghbali M, Baserisalehi M, Ghane M. Distribution of Virulence Genes in Moraxella catarrhalis Isolated from Clinical Samples in the North of Iran, Iran Red Crescent Med J. Online ahead of Print ; 21(1):e84125. doi: 10.5812/ircmj.61381.

Copyright © 2019, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Augustyniak D, Seredynski R, McClean S, Roszkowiak J, Roszniowski B, Smith DL, et al. Virulence factors of Moraxella catarrhalis outer membrane vesicles are major targets for cross-reactive antibodies and have adapted during evolution. Sci Rep. 2018;8(1):4955. doi: 10.1038/s41598-018-23029-7. [PubMed: 29563531]. [PubMed Central: PMC5862889].
  • 2. Prashanth HV, Dominic Saldanha RM, Shenoy S. Moraxella catarrhalis–A rediscovered pathogens. Int J Biol Med Res. 2011;2(4):979-81.
  • 3. Eltaib M, Elrhman A, Abdelhakam H, Khalid I, Abdelhalim A. Frequency of Moraxella catarrhalis from patients with lower respiratory tract infections in Khartoum state, Sudan. World J Pharma Res. 2015;4(5):2286-93.
  • 4. Liu G, Gradstedt H, Ermert D, Englund E, Singh B, Su YC, et al. Moraxella catarrhalis evades host innate immunity via targeting cartilage oligomeric matrix protein. J Immunol. 2016;196(3):1249-58. doi: 10.4049/jimmunol.1502071. [PubMed: 26712944].
  • 5. de Vries SP, Eleveld MJ, Hermans PW, Bootsma HJ. Characterization of the molecular interplay between Moraxella catarrhalis and human respiratory tract epithelial cells. PLoS One. 2013;8(8). e72193. doi: 10.1371/journal.pone.0072193. [PubMed: 23936538]. [PubMed Central: PMC3735583].
  • 6. Aebi C. Moraxella catarrhalis-pathogen or commensal? Adv Exp Med Biol. 2011;697:107-16.
  • 7. Verhaegh SJ, Snippe ML, Levy F, Verbrugh HA, Jaddoe VW, Hofman A, et al. Colonization of healthy children by Moraxella catarrhalis is characterized by genotype heterogeneity, virulence gene diversity and co-colonization with Haemophilus influenzae. Microbiology. 2011;157(Pt 1):169-78. doi: 10.1099/mic.0.042929-0. [PubMed: 20847012].
  • 8. Sillanpaa S, Oikarinen S, Sipila M, Kramna L, Rautiainen M, Huhtala H, et al. Moraxella catarrhalis might be more common than expected in acute otitis media in young finnish children. J Clin Microbiol. 2016;54(9):2373-9. doi: 10.1128/JCM.01146-16. [PubMed: 27413187]. [PubMed Central: PMC5005485].
  • 9. Verhaegh SJ, Streefland A, Dewnarain JK, Farrell DJ, van Belkum A, Hays JP. Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002. Microbiology. 2008;154(Pt 4):1178-84. doi: 10.1099/mic.0.2007/015057-0. [PubMed: 18375810].
  • 10. Hays JP, van Selm S, Hoogenboezem T, Estevao S, Eadie K, van Veelen P, et al. Identification and characterization of a novel outer membrane protein (OMP J) of Moraxella catarrhalis that exists in two major forms. J Bacteriol. 2005;187(23):7977-84. doi: 10.1128/JB.187.23.7977-7984.2005. [PubMed: 16291671]. [PubMed Central: PMC1291255].
  • 11. Berner R, Schumacher RF, Brandis M, Forster J. Colonization and infection with Moraxella catarrhalis in childhood. Eur J Clin Microbiol Infect Dis. 1996;15(6):506-9. [PubMed: 8839647].
  • 12. Masuda K, Masuda R, Nishi J, Tokuda K, Yoshinaga M, Miyata K. Incidences of nasopharyngeal colonization of respiratory bacterial pathogens in Japanese children attending day-care centers. Pediatr Int. 2002;44(4):376-80. doi: 10.1046/j.1442-200X.2002.01587.x. [PubMed: 12139560].
  • 13. Abuhammour WM, Abdel-Haq NM, Asmar BI, Dajani AS. Moraxella catarrhalis bacteremia: A 10-year experience. South Med J. 1999;92(11):1071-4. doi: 10.1097/00007611-199911000-00005. [PubMed: 10586832].
  • 14. Heiniger N, Spaniol V, Troller R, Vischer M, Aebi C. A reservoir of Moraxella catarrhalis in human pharyngeal lymphoid tissue. J Infect Dis. 2007;196(7):1080-7. doi: 10.1086/521194. [PubMed: 17763332].
  • 15. Bernhard S, Spaniol V, Aebi C. Molecular pathogenesis of infections caused by Moraxella catarrhalis in children. Swiss Med Wkly. 2012;142:w13694. doi: 10.4414/smw.2012.13694. [PubMed: 23136074].
  • 16. Gergova RT, Petrova G, Gergov S, Minchev P, Mitov I, Strateva T. Microbiological features of upper respiratory tract infections in bulgarian children for the period 1998-2014. Balkan Med J. 2016;33(6):675-80. doi: 10.5152/balkanmedj.2016.150116. [PubMed: 27994923]. [PubMed Central: PMC5156459].
  • 17. Perez AC, Murphy TF. A Moraxella catarrhalis vaccine to protect against otitis media and exacerbations of COPD: An update on current progress and challenges. Hum Vaccin Immunother. 2017;13(10):2322-31. doi: 10.1080/21645515.2017.1356951. [PubMed: 28853985]. [PubMed Central: PMC5647992].
  • 18. Murphy TF, Parameswaran GI. Moraxella catarrhalis, a human respiratory tract pathogen. Clin Infect Dis. 2009;49(1):124-31. doi: 10.1086/599375. [PubMed: 19480579].
  • 19. Ramana BV, Chaudhury A. Antibiotic sensitivity pattern of Moraxella catarrhalis at a tertiary care hospital. Int J of Pharm Life Sci. 2012;3(7):1805-6.
  • 20. Mohager MO, Hassan EI, Omer EI, Elmekki MA. Molecular detection of BRO β-lactamase gene of Moraxella catarrhalis isolated from Sudanese patients. Ann Trop Med Pub Health. 2013;6(441-5).
  • 21. Ramadan MO, Ibrahim IS, Shaheen AM, Ali WE. Significance of Moraxella catarrhalis as a causative organism of lower respiratory tract infections. Egypt J Chest Dis Tuberc. 2017;66(3):459-64. doi: 10.1016/j.ejcdt.2016.05.011.
  • 22. Singh B, Alvarado-Kristensson M, Johansson M, Hallgren O, Westergren-Thorsson G, Morgelin M, et al. The respiratory pathogen Moraxella catarrhalis targets collagen for maximal adherence to host tissues. MBio. 2016;7(2). e00066. doi: 10.1128/mBio.00066-16. [PubMed: 27006460]. [PubMed Central: PMC4807357].
  • 23. Mollenkvist A, Nordstrom T, Hallden C, Christensen JJ, Forsgren A, Riesbeck K. The Moraxella catarrhalis immunoglobulin D-binding protein MID has conserved sequences and is regulated by a mechanism corresponding to phase variation. J Bacteriol. 2003;185(7):2285-95. doi: 10.1128/JB.185.7.2285-2295.2003. [PubMed: 12644500]. [PubMed Central: PMC151486].
  • 24. Bootsma HJ, van Dijk H, Vauterin P, Verhoef J, Mooi FR. Genesis of BRO beta-lactamase-producing Moraxella catarrhalis: Evidence for transformation-mediated horizontal transfer. Mol Microbiol. 2000;36(1):93-104. doi: 10.1046/j.1365-2958.2000.01828.x. [PubMed: 10760166].
  • 25. Mitov IG, Gergova RT, Ouzounova-Raykova VV. Distribution of genes encoding virulence factors ompB2, ompCD, ompE, beta-lactamase and serotype in pathogenic and colonizing strains of Moraxella catarrhalis. Arch Med Res. 2010;41(7):530-5. doi: 10.1016/j.arcmed.2010.10.003. [PubMed: 21167392].
  • 26. Liu YL, Xiao M, Cheng JW, Xu HP, Xu ZP, Ye S, et al. Moraxella catarrhalis Macrolide-Resistant Isolates Are Highly Concentrated in Two MLST Clonal Complexes -CCN10 and CC363. Front Microbiol. 2017;8:201. doi: 10.3389/fmicb.2017.00201. [PubMed: 28239374]. [PubMed Central: PMC5300973].
  • 27. Blakeway LV, Tan A, Peak IRA, Seib KL. Virulence determinants of Moraxella catarrhalis: Distribution and considerations for vaccine development. Microbiology. 2017;163(10):1371-84. doi: 10.1099/mic.0.000523. [PubMed: 28893369].
  • 28. Meier PS, Troller R, Grivea IN, Syrogiannopoulos GA, Aebi C. The outer membrane proteins UspA1 and UspA2 of Moraxella catarrhalis are highly conserved in nasopharyngeal isolates from young children. Vaccine. 2002;20(13-14):1754-60. doi: 10.1016/S0264-410X(02)00030-0. [PubMed: 11906762].
  • 29. Lafontaine ER, Cope LD, Aebi C, Latimer JL, McCracken GH Jr, Hansen EJ. The UspA1 protein and a second type of UspA2 protein mediate adherence of Moraxella catarrhalis to human epithelial cells in vitro. J Bacteriol. 2000;182(5):1364-73. doi: 10.1128/JB.182.5.1364-1373.2000. [PubMed: 10671460]. [PubMed Central: PMC94425].
  • 30. Broides A, Dagan R, Greenberg D, Givon-Lavi N, Leibovitz E. Acute otitis media caused by Moraxella catarrhalis: Epidemiologic and clinical characteristics. Clin Infect Dis. 2009;49(11):1641-7. doi: 10.1086/647933. [PubMed: 19886799].
  • 31. Jordan KL, Berk SH, Berk SL. A comparison of serum bactericidal activity and phenotypic characteristics of bacteremic, pneumonia-causing strains, and colonizing strains of Branhamella catarrhalis. Am J Med. 1990;88(5A):28S-32S. doi: 10.1016/0002-9343(90)90258-F. [PubMed: 2111090].
  • 32. Pearson MM, Laurence CA, Guinn SE, Hansen EJ. Biofilm formation by Moraxella catarrhalis in vitro: Roles of the UspA1 adhesin and the Hag hemagglutinin. Infect Immun. 2006;74(3):1588-96. doi: 10.1128/IAI.74.3.1588-1596.2006. [PubMed: 16495530]. [PubMed Central: PMC1418653].

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