The Antimicrobial Effect of Lactobacillus Casei Culture Supernatant Against Multiple Drug Resistant Clinical Isolates of Shigella Sonnei and Shigella Flexneri in Vitro

AUTHORS

Reza Mirnejad 1 , Ali Reza Vahdati 2 , Jamal Rashidiani 3 , Mohammad Erfani 4 , Vahhab Piranfar 4 , *

1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran

2 Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland

3 Nano Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran

4 Department of Microbiology, Tonekabon Branch, Islamic Azad University of Tonekabon, Tonekabon, IR Iran

How to Cite: Mirnejad R, Vahdati A R, Rashidiani J, Erfani M, Piranfar V. The Antimicrobial Effect of Lactobacillus Casei Culture Supernatant Against Multiple Drug Resistant Clinical Isolates of Shigella Sonnei and Shigella Flexneri in Vitro, Iran Red Crescent Med J. Online ahead of Print ; 15(2):122-6. doi: 10.5812/ircmj.7454.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 15 (2); 122-6
Published Online: February 4, 2013
Article Type: Research Article
Received: July 25, 2012
Accepted: January 7, 2013
Crossmark

Crossmark

CHEKING

READ FULL TEXT
Abstract

Backgrounds: Shigellosis remains an important public health problem in developing countries with S. sonnei and S. flexneri in US, Europe and in Asian countries being of importance.

Objectives: This study evaluates the protective effect of Lactobacillus casei cell-free culture supernatants (CFCS) against multiple drug resistance (MDR) clinical samples of Shigella sonnei and Shigella flexneri in vitro.

Materials and Methods: S. sonnei and S .flexneri was identified by common microbiological and serological methods. Antibiogram with 18 antibiotics were tested for 34 positive cultures by disc diffusion method. The Samples showed considerable resistance to antibiotics. Antimicrobial effects of CFCS were tested against S. sonnei and S. flexneri by agar-well assay and broth micro dilution methods. In addition, the antimicrobial activity remained active treatment after adjust pH 7, adding Proteinase K and heating for L. casei.

Results: The results implicate that L. casei strongly inhibits the development of pathogen samples. In contrast, via the disc diffusion method 4 out of 18 antibiogram have shown complete resistance against the pathogen samples. In addition, the natures of antimicrobial properties have been tested in different conditions such as various pH, temperature and presence of proteinase K. The MIC50 (minimum inhibitory concentration) and MIC90 of CFCS of L. casei were determined, for S. sonnei were 2.25 and 10.5, for S .flexneri were 5.25 and 5.25 respectively. The results have shown a significant resistance pattern by these four antibiotics in this case.

Conclusions: The data indicates that. L. casei highly resistant against to antibiotics, heat, Proteinase K and so many activities against MDR Shigella pathogenic strains . L. casei is the best probiotics candidate.

Keywords

Lactobacillus Casei Shigella Flexneri Shigella Sonnei

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: