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Influenza Virus-like Particle Containing Two Different Subtypes of Hemagglutinin Confers Protection in Mice Against Lethal Challenge With A/PR8 (H1N1) and A/HK (H3N2) Viruses

AUTHORS

Farhad Rezaei 1 , Abbas Mirshafiey 2 , Shohreh Shahmahmoodi 1 , Zabihollah Shoja 1 , Nastaran Ghavami 1 , Talat Mokhtari-Azad 1 , *

1 Department of Virology, School of Public Health, Tehran University of Medical Sciences, mokhtari@tums.ac.ir, IR Iran

2 Department of Immunology, School of Public Health, Tehran University of Medical Sciences, IR Iran

How to Cite: Rezaei F, Mirshafiey A, Shahmahmoodi S, Shoja Z, Ghavami N, et al. Influenza Virus-like Particle Containing Two Different Subtypes of Hemagglutinin Confers Protection in Mice Against Lethal Challenge With A/PR8 (H1N1) and A/HK (H3N2) Viruses, Iran Red Crescent Med J. Online ahead of Print ; 15(1):75-82. doi: 10.5812/ircmj.6252.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 15 (1); 75-82
Published Online: January 4, 2013
Article Type: Research Article
Received: May 7, 2012
Accepted: September 23, 2012
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Abstract

Background: Preventing the seasonal or pandemic outbreak of influenza can be powerful and cost-effective.

Objectives: In this study, we constructed a novel virus-like particle (VLP) platform that contains two hemagglutinin (HA) subtypes and evaluated immunogenicity of constructed VLP in mice.

Materials and Methods: This recombinant candidate vaccine model resulted in the expression of two HAs of H1N1 and H3N2 subtypes co-localized within a VLP. Following infection of insect cells with recombinant baculovirus co-expressing H1, H3 and M1 proteins, VLPs with size of 80120 nm were self-assembled, budding, and released into the insect culture medium. The resulting VLPs which contained two different subtypes of hemagglutinin were purified by ultracentrifugation. The immunogenicity of VLPs was evaluated in mice following immunization.

Results: Our data showed that vaccination using VLPs elicited robust levels of serum IgG, and viral neutralizing antibodies against A/PR8 (H1N1) and A/HK (H3N2) viruses. Following challenge with lethal dose of A/PR8 (H1N1) and A/HK (H3N2), vaccinated mice were protected, displaying no sign of weight loss and mortality compared to non-vaccinated control mice.

Conclusions: VLPs can serve as a promising vaccination strategy to control influenza virus.

Keywords

Orthomyxoviridae Virus-like particles Influenza Vaccines

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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