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Tissue Engineering Using Human Mineralized Bone Xenograft and Bone Marrow Mesenchymal Stem Cells Allograft in Healing of Tibial Fracture of Experimental Rabbit Model

AUTHORS

J Ai 1 , S Ebrahimi 2 , A Khoshzaban 2 , TS Jafarzadeh Kashi 2 , D Mehrabani 3 , * , 1

AUTHORS INFORMATION

1 Department of Tissue Engineering and Cell Therapy, School of Advanced Medical Technologies, and Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran and Stem Cell and Transgenic Technology Research Ce

2 Department of Biology, Faculty of Sciences, University of Tarbiat Moallem, Iran

3 Assistant Professor of Pathology, Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Mother and Child Hospital, mehrabad@sums.ac.ir, Iran

How to Cite: Ai J, Ebrahimi S, Khoshzaban A, Jafarzadeh Kashi T, Mehrabani D, et al. Tissue Engineering Using Human Mineralized Bone Xenograft and Bone Marrow Mesenchymal Stem Cells Allograft in Healing of Tibial Fracture of Experimental Rabbit Model, Iran Red Crescent Med J. Online ahead of Print ; 14(2):96-103.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 14 (2); 96-103
Article Type: Research Article
Received: September 6, 2011
Accepted: December 22, 2011

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Abstract

Background: Bone healing and its reconstruction in fractures, especially in long bones are of particular importance in regenerative medicine. This study compares the bone healing rate after a human xenograft of mineralized bone and together with an allograft of bone marrow mesenchymal stem cells (MSCs) in an experimental tibial bone fracture rabbit model.

 

Methods:In fall 2009, twenty New Zealand white rabbits were randomly divided into 2 equal groups. In both groups, a 5 mm segmental defect was created in the right tibia. In group A, a scaffold pin was seeded with allogenic rabbit MSCs and was placed in the defect area and in group B, the defect was filled with an unseeded pin human mineralized bone xenograft. An untreated defect was induced in the left tibia of all animals serving as the control. After 4-8 weeks, the segmental defects were histologically evaluated and also by a compressive test.

 

Results: In groups A and B, healing and formation of new bony tissue were significantly more than the control group and with a significant less inflammation.

 

Conclusion:Tissue engineering of mineralized bone xenograft and MSCs allograft may be significant steps in bone healing and regenerative medicine.

Keywords

Bone Xenograft Allograft Mesenchymal stem cells Healing Rabbit

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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