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Comparison of ELISA and Three Rapid HCG Dipsticks in Diagnosis of Premature Rupture of Membranes

AUTHORS

N Kariman 1 , * , M Hedayati 2 , Z Taheri 2 , M Fallahian 2 , S Salehpoor 2 , SH Alavi Majd 2

AUTHORS INFORMATION

1 Faculty of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, n_kariman@sbmu.ac.ir, Tehran, Iran

2 Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

How to Cite: Kariman N, Hedayati M, Taheri Z, Fallahian M, Salehpoor S, et al. Comparison of ELISA and Three Rapid HCG Dipsticks in Diagnosis of Premature Rupture of Membranes, Iran Red Crescent Med J. Online ahead of Print ; 13(6):415-419.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 13 (6); 415-419
Article Type: Research Article
Received: September 20, 2010
Accepted: December 12, 2010

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Abstract

Background: The importance of accurate diagnosis of premature rupture of membranes (PROM) is quite apparent while trying to diminish false negative or positive results as much as possible. This study compares ------ (ELISA) and three rapid human chorionic gonadothropin (HCG) dipsticks in diagnosis of premature rupture of membranes.

Methods:  During 2008-2009, 181 pregnant women with single pregnancy from 14 to 41 weeks of gestation who referred to Ayatollah Taleghani Hospital in Tehran, Iran were divided into two groups, 91 patients with PROM and 90 controls with matched gestational weeks. All patients underwent speculum examination for cervicovaginal washing fluid, HCG three rapid tests and ELISA.

Results: The HCG concentration of vaginal fluid was significantly different between the two groups. Using receiver operating characteristic (ROC) curve and determining the threshold as 19 mIU/mL for HCG by ELISA method, the sensitivity was 94.5%; specificity, 91%; positive predictive value, 91.5%; negative predicted value, 94.2% and accuracy was 92.2%. In rapid diagnostic test, the most sensitivity was for ----- (ACON) and the most specificity for ----- (DIMA). Comparing the four methods, DIMA strip showed the highest accuracy and the highest value in early diagnosis of ROM.

Conclusion: The reliability of three rapid diagnostic tests in diagnosis of ROM in cervicovaginal discharge was acceptable.

Keywords

Premature rupture of membranes Human chorionic gonadotropin Cervicovaginal discharge Rapid test strip ELISA

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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