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Expansion of Human Marrow Derived Mesenchymal Stem Cells and their Transdifferentiation Potential

AUTHORS

M Ayatollahi 1 , M Kabir Salmani 2 , * , M Soleimani 3 , B Geramizadeh 4 , MH Sanati 5 , M Gardaneh 5 , SZ Tabei 4

AUTHORS INFORMATION

1 Stem Cell and Transgenic Technology Research Center, Transplant Research Center, Shiraz University of Medical Sciences, Fars, Iran

2 Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, PO Box: 14965 -161, kabirs_m@yahoo.com, Tehran, Iran

3 Department of Hematology, Tarbiat Modares University, Tehran, Iran

4 Transplant Research Center, Shiraz University of Medical Sciences, Iran

5 National Institute of Genetic Engineering and Biotechnology, Tarbiat Modares University, Iran

How to Cite: Ayatollahi M, Kabir Salmani M, Soleimani M, Geramizadeh B, Sanati M, et al. Expansion of Human Marrow Derived Mesenchymal Stem Cells and their Transdifferentiation Potential, Iran Red Crescent Med J. Online ahead of Print ; 12(4):446-452.

ARTICLE INFORMATION

Iranian Red Crescent Medical Journal: 12 (4); 446-452
Article Type: Research Article
Received: July 10, 2009
Accepted: November 30, 2009

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Abstract

Background: The ability of mesenchymal stem cells (MSCs) to differentiate into other cell types makes these cells an attractive therapeutic tool for cell transplantation. In order to provide a source of human MSCs for autologus cell-based therapy, we have expanded MSCs from the bone marrow and analyzed the biological identities and transdifferentiation potential.

 

Methods: The bone marrow of healthy donors was aspirated from the iliac crest. The adjacent cells expanded rapidly and maintained with periodic passages until a relatively homogeneous population was established. The identification of these cells was carried out by differentiation potential into the osteocytes and adipocytes. Transdifferentiation of human MSCs into hepatocyte-like cells was undertaken in response to a specific culture condition.

 

Results: The differentiation of MSCs into osteoblast is determined by deposition of a mineralized extracellular matrix. Adipocytes are identified by their morphology and staining. Hepatic cells were demonstrated in vitro functions characteristic of liver cells.

 

Conclusion: We have defined conditions under which human MSCs can be isolated and expanded from human bone marrow. These cells can be amplified about 108-fold in 6 weeks, and are capable of transdifferentiation into the cells of another developmental lineage.

Keywords

Mesenchymal stem cells Human Differentiation Bone marrow

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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