Iranian Red Crescent Medical Journal

Published by: Kowsar

Improvement of Th1/Th2 and Th1/Treg Imbalances by Adjutants CPG, MPLA and BCG in a Model of Acute Asthma Induced By Allergen Derp2 in BALB/c Mice

Vahid Mohammadi-Shahrokhi 1 , Abbas Rezaei 1 , Alireza Andalib 1 , Amir Rahnama 2 , Abdollah Jafarzadeh 3 , ** and Nahid Eskandari 1 , **
Authors Information
1 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Pathology, School of Medicine, Rafsanjan University of Medical Sciences Rafsanjan, Iran
3 Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Corresponding Authors:
Article information
  • Iranian Red Crescent Medical Journal: March 01, 2017, 19 (3); e41114
  • Published Online: October 16, 2016
  • Article Type: Research Article
  • Received: July 27, 2016
  • Revised: August 23, 2016
  • Accepted: September 27, 2016
  • DOI: 10.5812/ircmj.41114

To Cite: Mohammadi-Shahrokhi V, Rezaei A, Andalib A, Rahnama A, Jafarzadeh A, et al. Improvement of Th1/Th2 and Th1/Treg Imbalances by Adjutants CPG, MPLA and BCG in a Model of Acute Asthma Induced By Allergen Derp2 in BALB/c Mice, Iran Red Crescent Med J. 2017 ; 19(3):e41114. doi: 10.5812/ircmj.41114.

Copyright © 2016, Iranian Red Crescent Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Salehi S. The role of syk in airway hyperresponsiveness and remodeling in house dust mite induced murine models of allergic airways inflammation 2013;
  • 2. Matsubara S, Koya T, Takeda K, Joetham A, Miyahara N, Pine P, et al. Syk activation in dendritic cells is essential for airway hyperresponsiveness and inflammation. Am J Respir Cell Mol Biol. 2006; 34(4): 426-33[DOI][PubMed]
  • 3. Arbes SJ, Gergen PJ, Vaughn B, Zeldin DC. Asthma cases attributable to atopy: results from the Third National Health and Nutrition Examination Survey. J Allergy Clin Immunol. 2007; 120(5): 1139-45[DOI][PubMed]
  • 4. Gregory LG, Lloyd CM. Orchestrating house dust mite-associated allergy in the lung. Trends Immunol. 2011; 32(9): 402-11[DOI][PubMed]
  • 5. Calderon MA, Kleine-Tebbe J, Linneberg A, De Blay F, Hernandez Fernandez de Rojas D, Virchow JC, et al. House Dust Mite Respiratory Allergy: An Overview of Current Therapeutic Strategies. J Allergy Clin Immunol Pract. 2015; 3(6): 843-55[DOI][PubMed]
  • 6. Wang X, Yang Q, Wang P, Luo L, Chen Z, Bin L, et al. Derp2-mutant gene vaccine inhibits airway inflammation and up-regulates Toll-like receptor 9 in an allergic asthmatic mouse model. Asian Pacific J Allergy Immunol. 2010; 28(4): 287
  • 7. Genc S, Eroglu H, Kucuksezer UC, Aktas-Cetin E, Gelincik A, Ustyol-Aycan E, et al. The decreased CD4+CD25+ FoxP3+ T cells in nonstimulated allergic rhinitis patients sensitized to house dust mites. J Asthma. 2012; 49(6): 569-74[DOI][PubMed]
  • 8. Tong P, Wesemann DR. Molecular mechanisms of IgE class switch recombination. IgE Antibodies: Generation and Function. 2015; : 21-37
  • 9. Bienvenu J, Doche C, Gutowski MC, Lenoble M, Lepape A, Perdrix JP. Production of proinflammatory cytokines and cytokines involved in the TH1/TH2 balance is modulated by pentoxifylline. J Cardiovasc Pharmacol. 1995; 25 Suppl 2-4[PubMed]
  • 10. Kim DH, Sohn JH, Park HJ, Lee JH, Park JW, Choi JM. CpG Oligodeoxynucleotide Inhibits Cockroach-Induced Asthma via Induction of IFN-gamma(+) Th1 Cells or Foxp3(+) Regulatory T Cells in the Lung. Allergy Asthma Immunol Res. 2016; 8(3): 264-75[DOI][PubMed]
  • 11. Santeliz JV, Van Nest G, Traquina P, Larsen E, Wills-Karp M. Amb a 1-linked CpG oligodeoxynucleotides reverse established airway hyperresponsiveness in a murine model of asthma. J Allergy Clin Immunol. 2002; 109(3): 455-62[PubMed]
  • 12. Dubensky TJ, Reed SG. Adjuvants for cancer vaccines. Semin Immunol. 2010; 22(3): 155-61[DOI][PubMed]
  • 13. Joshi J, Kaur S. To investigate the therapeutic potential of immunochemotherapy with cisplatin + 78 kDa + MPL-A against Leishmania donovani in BALB/c mice. Parasite Immunol. 2014; 36(1): 3-12[DOI][PubMed]
  • 14. Habibi M, Asadi Karam MR, Bouzari S. Evaluation of the effect of MPL and delivery route on immunogenicity and protectivity of different formulations of FimH and MrpH from uropathogenic Escherichia coli and Proteus mirabilis in a UTI mouse model. Int Immunopharmacol. 2015; 28(1): 70-8[DOI][PubMed]
  • 15. Heldwein KA, Liang MD, Andresen TK, Thomas KE, Marty AM, Cuesta N, et al. TLR2 and TLR4 serve distinct roles in the host immune response against Mycobacterium bovis BCG. J Leukoc Biol. 2003; 74(2): 277-86[PubMed]
  • 16. Akkoc T, Aydogan M, Yildiz A, Karakoc-Aydiner E, Eifan A, Keles S, et al. Neonatal BCG vaccination induces IL-10 production by CD4+ CD25+ T cells. Pediatr Allergy Immunol. 2010; 21(7): 1059-63[DOI][PubMed]
  • 17. Boer MC, Prins C, van Meijgaarden KE, van Dissel JT, Ottenhoff TH, Joosten SA. Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults. Clin Vaccine Immunol. 2015; 22(7): 778-88[DOI][PubMed]
  • 18. Verwaerde C, Debrie AS, Dombu C, Legrand D, Raze D, Lecher S, et al. HBHA vaccination may require both Th1 and Th17 immune responses to protect mice against tuberculosis. Vaccine. 2014; 32(47): 6240-50[DOI][PubMed]
  • 19. Gu D, Chen W, Mi Y, Gong X, Luo T, Bao L. The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice. Acta Biochim Biophys Sin (Shanghai). 2016; 48(4): 385-90[DOI][PubMed]
  • 20. Ballester M, Jeanbart L, de Titta A, Nembrini C, Marsland BJ, Hubbell JA, et al. Nanoparticle conjugation enhances the immunomodulatory effects of intranasally delivered CpG in house dust mite-allergic mice. Sci Rep. 2015; 5: 14274[DOI][PubMed]
  • 21. Ou J, Shi W, Xu Y, Tao Z. Intranasal immunization with DNA vaccine coexpressing Der p 1 and ubiquitin in an allergic rhinitis mouse model. Annals Allergy, Asthma Immunol. 2014; 113(6): 658-65
  • 22. Lagranderie M, Nahori MA, Balazuc AM, Kiefer-Biasizzo H, Lapa e Silva JR, Milon G, et al. Dendritic cells recruited to the lung shortly after intranasal delivery of Mycobacterium bovis BCG drive the primary immune response towards a type 1 cytokine production. Immunology. 2003; 108(3): 352-64[PubMed]
  • 23. Rolland-Debord C, Lair D, Roussey-Bihouee T, Hassoun D, Evrard J, Cheminant MA, et al. Block copolymer/DNA vaccination induces a strong allergen-specific local response in a mouse model of house dust mite asthma. PLoS One. 2014; 9(1): 85976[DOI][PubMed]
  • 24. Hiroi T, Kaminuma O, Takaiwa F. Vaccination with transgenic rice seed expressing mite allergen: a new option for asthma sufferers? Expert Rev Vaccines. 2011; 10(9): 1249-51[DOI][PubMed]
  • 25. Suzuki K, Kaminuma O, Yang L, Takai T, Mori A, Umezu-Goto M, et al. Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen-specific oral tolerance without bystander suppression. Plant Biotechnol J. 2011; 9(9): 982-90[DOI][PubMed]
  • 26. Starkhammar M, Larsson O, Kumlien Georen S, Leino M, Dahlen SE, Adner M, et al. Toll-like receptor ligands LPS and poly (I:C) exacerbate airway hyperresponsiveness in a model of airway allergy in mice, independently of inflammation. PLoS One. 2014; 9(8): 104114[DOI][PubMed]
  • 27. Patent US3598122 1982;
  • 28. Li HB, Zhang JY, He YF, Chen L, Li B, Liu KY, et al. Systemic immunization with an epitope-based vaccine elicits a Th1-biased response and provides protection against Helicobacter pylori in mice. Vaccine. 2012; 31(1): 120-6[DOI][PubMed]
  • 29. Ito T, Takii T, Maruyama M, Hayashi D, Wako T, Asai A, et al. Effectiveness of BCG vaccination to aged mice. Immun Ageing. 2010; 7: 12[DOI][PubMed]
  • 30. Fernandez I, Pena A, Del Teso N, Perez V, Rodriguez-Cuesta J. Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retroorbital plexus. J Am Assoc Lab Anim Sci. 2010; 49(2): 202-6[PubMed]
  • 31. Duechs MJ, Brunt JE, Gantner F, Erb KJ. Adjuvant effects of different TLR agonists on the induction of allergen-specific Th2 responses. Open J Immunol. 2012; 2(1): 17
  • 32. Mackenzie KJ, Nowakowska DJ, Leech MD, McFarlane AJ, Wilson C, Fitch PM, et al. Effector and central memory T helper 2 cells respond differently to peptide immunotherapy. Proc Natl Acad Sci U S A. 2014; 111(8): 784-93[DOI][PubMed]
  • 33. Duchs M, der Promotionskommission M. Effects of Toll-like receptor agonists on the pathogenesis of atopic asthma in mice 2011;
  • 34. Coleman MM, Keane J, Mills KH. Editorial: Tregs and BCG--dangerous liaisons in TB. J Leukoc Biol. 2010; 88(6): 1067-9[DOI][PubMed]
  • 35. Wu CJ, Chou HW, Liou CJ, Shen JJ, Wang LC, Kuo ML. Prophylactic vaccination with adjuvant monophosphoryl lipid a prevents Th2-mediated murine asthmatic responses. J Asthma. 2013; 50(4): 327-33[DOI][PubMed]
  • 36. Deng Y, Li W, Luo Y, Wang LJ, Xie XH, Luo J, et al. Inhibition of IFN-gamma promotes anti-asthma effect of Mycobacterium bovis Bacillus Calmette-Guerin neonatal vaccination: a murine asthma model. Vaccine. 2014; 32(18): 2070-8[DOI][PubMed]
  • 37. Soleimani M, Rafinejad J. House dust mite contamination in hotels and inns in Bandar Abbas, south of Iran. J Envi Health Sci Eng. 2008; 5(3): 207-10
  • 38. Kleinnijenhuis J, Quintin J, Preijers F, Benn CS, Joosten LA, Jacobs C, et al. Long-lasting effects of BCG vaccination on both heterologous Th1/Th17 responses and innate trained immunity. J Innate Immun. 2014; 6(2): 152-8[DOI][PubMed]
  • 39. Thomas WR, Hales BJ, Smith WA. House dust mite allergens in asthma and allergy. Trends Mol Med. 2010; 16(7): 321-8[DOI][PubMed]
  • 40. VanCott JL, Prada AE, McNeal MM, Stone SC, Basu M, Huffer BJ, et al. Mice develop effective but delayed protective immune responses when immunized as neonates either intranasally with nonliving VP6/LT(R192G) or orally with live rhesus rotavirus vaccine candidates. J Virol. 2006; 80(10): 4949-61[DOI][PubMed]
  • 41. Christe M, Rutti B, Brossard M. Cytokines (IL-4 and IFN-gamma) and antibodies (IgE and IgG2a) produced in mice infected with Borrelia burgdorferi sensu stricto via nymphs of Ixodes ricinus ticks or syringe inoculations. Parasitol Res. 2000; 86(6): 491-6[PubMed]
  • 42. Demir T, Canakci V, Erdem F, Atasever M, Kara C, Canakci CF. The effects of age and gender on gingival tissue and peripheral blood T-lymphocyte subsets: a study in mice. Immunol Invest. 2008; 37(2): 171-82[DOI][PubMed]
  • 43. Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development. 2012; 139(22): 4210-9[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments