Iranian Red Crescent Medical Journal

Published by: Kowsar

Evaluation of the Immunogenicity of Diphtheria Toxoid Conjugated to Salmonella Typhimurium-Derived OPS in a Mouse Model: A Potential Vaccine Candidate Against Salmonellosis

Vahid Amini 1 , Hossein Kazemian 2 , Jalil Kardan Yamchi 3 , Seifu Gizaw Feyisa 4 , Saeed Aslani 5 , Aref Shavalipour 6 , Hamidreza Houri 6 , Mohammadneshvan Hoorijani 7 , Mehrdad Halaji 8 and Hamid Heidari 8 , *
Authors Information
1 Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran
2 Department of Medical Microbiology, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Pathobiology, Division of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
4 Tehran University of Medical Sciences, International Campus (TUMS-IC), Tehran, IR Iran
5 Department of Immunology, Tehran University of Medical Sciences, Tehran, IR Iran
6 Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
7 Department of Microbiology, Kurdistan Science and Research Branch, Islamic Azad University, Sanandaj, IR Iran
8 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran
Article information
  • Iranian Red Crescent Medical Journal: July 01, 2016, 18 (7); e34135
  • Published Online: May 30, 2016
  • Article Type: Research Article
  • Received: October 28, 2015
  • Revised: November 30, 2015
  • Accepted: January 12, 2016
  • DOI: 10.5812/ircmj.34135

To Cite: Amini V, Kazemian H, Yamchi J K, Feyisa S G, Aslani S, et al. Evaluation of the Immunogenicity of Diphtheria Toxoid Conjugated to Salmonella Typhimurium-Derived OPS in a Mouse Model: A Potential Vaccine Candidate Against Salmonellosis, Iran Red Crescent Med J. 2016 ; 18(7):e34135. doi: 10.5812/ircmj.34135.

Copyright © 2016, Iranian Red Crescent Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Thiennimitr P, Winter SE, Baumler AJ. Salmonella, the host and its microbiota. Curr Opin Microbiol. 2012; 15(1): 108-14[DOI][PubMed]
  • 2. Fang FC. Antimicrobial reactive oxygen and nitrogen species: concepts and controversies. Nat Rev Microbiol. 2004; 2(10): 820-32[DOI][PubMed]
  • 3. Wong MH, Yan M, Chan EW, Biao K, Chen S. Emergence of clinical Salmonella enterica serovar Typhimurium isolates with concurrent resistance to ciprofloxacin, ceftriaxone, and azithromycin. Antimicrob Agents Chemother. 2014; 58(7): 3752-6[DOI][PubMed]
  • 4. Kazemian H, Ghafourian S, Heidari H, Amiri P, Yamchi JK, Shavalipour A, et al. Antibacterial, anti-swarming and anti-biofilm formation activities of Chamaemelum nobile against Pseudomonas aeruginosa. Rev Soc Bras Med Trop. 2015; 48(4): 432-6[DOI][PubMed]
  • 5. Kazemian H, Haeili M, Kardan Yamchi J, Rezaei F, Gizaw Feyisa S, Zahednamazi F, et al. Antimycobacterial activity of linezolid against multidrug-resistant and extensively drug-resistant strains of Mycobacterium tuberculosis in Iran. Int J Antimicrob Agents. 2015; 45(6): 668-70[DOI][PubMed]
  • 6. Rahimi S, Shiraz ZM, Salehi TZ, Torshizi MAK, Grimes JL. Prevention of salmonella infection in poultry by specific egg-derived antibody. Int J Poult Sci. 2007; 6(4): 230-5[DOI]
  • 7. Zhao S, Qaiyumi S, Friedman S, Singh R, Foley SL, White DG, et al. Characterization of Salmonella enterica serotype newport isolated from humans and food animals. J Clin Microbiol. 2003; 41(12): 5366-71[PubMed]
  • 8. Butler BA. A commercially available siderophore-receptor and porin-based vaccine reduced the prevalence of escherichia coli o157: H7 in the feces of beef cattle under field conditions in 10 commercial feedlots [Dissertation]. 2012;
  • 9. Ledesma MA, Ochoa SA, Cruz A, Rocha-Ramirez LM, Mas-Oliva J, Eslava CA, et al. The hemorrhagic coli pilus (HCP) of Escherichia coli O157:H7 is an inducer of proinflammatory cytokine secretion in intestinal epithelial cells. PLoS One. 2010; 5(8): 12127[DOI][PubMed]
  • 10. Marathe SA, Lahiri A, Negi VD, Chakravortty D. Typhoid fever & vaccine development: a partially answered question. Indian J Med Res. 2012; 135: 161-9[PubMed]
  • 11. Plotkin SA. Correlates of protection induced by vaccination. Clin Vaccine Immunol. 2010; 17(7): 1055-65[DOI][PubMed]
  • 12. Aguilar JC, Rodriguez EG. Vaccine adjuvants revisited. Vaccine. 2007; 25(19): 3752-62[DOI][PubMed]
  • 13. Cui C, Carbis R, An SJ, Jang H, Czerkinsky C, Szu SC, et al. Physical and chemical characterization and immunologic properties of Salmonella enterica serovar typhi capsular polysaccharide-diphtheria toxoid conjugates. Clin Vaccine Immunol. 2010; 17(1): 73-9[DOI][PubMed]
  • 14. Izquierdo L, Abitiu N, Coderch N, Hita B, Merino S, Gavin R, et al. The inner-core lipopolysaccharide biosynthetic waaE gene: function and genetic distribution among some Enterobacteriaceae. Microbiology. 2002; 148: 3485-96[DOI][PubMed]
  • 15. Najafzadeh F, Shapouri R, Rahnema M, Rokhsartalab Azar S, Kianmehr A. Pseudomonas aeruginosa PAO-1 Lipopolysaccharide-Diphtheria Toxoid Conjugate Vaccine: Preparation, Characterization and Immunogenicity. Jundishapur J Microbiol. 2015; 8(6): 17712[DOI][PubMed]
  • 16. Simon R, Levine MM. Glycoconjugate vaccine strategies for protection against invasive Salmonella infections. Hum Vaccin Immunother. 2012; 8(4): 494-8[DOI][PubMed]
  • 17. Theilacker C, Coleman FT, Mueschenborn S, Llosa N, Grout M, Pier GB. Construction and characterization of a Pseudomonas aeruginosa mucoid exopolysaccharide-alginate conjugate vaccine. Infect Immun. 2003; 71(7): 3875-84[PubMed]
  • 18. Tsuboi T, Takeo S, Iriko H, Jin L, Tsuchimochi M, Matsuda S, et al. Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infect Immun. 2008; 76(4): 1702-8[DOI][PubMed]
  • 19. Rao AR, Dayananda C, Sarada R, Shamala TR, Ravishankar GA. Effect of salinity on growth of green alga Botryococcus braunii and its constituents. Bioresour Technol. 2007; 98(3): 560-4[DOI][PubMed]
  • 20. Rauber RH, Perlin VJ, Fin CD, Mallmann AL, Miranda DP, Giacomini LZ, et al. Interference of salmonella typhimurium lipopolysaccharide on performance and biological parameters of broiler chickens. Rev Bras Cienc Avic. 2014; 16(1): 77-81[DOI]
  • 21. Park CY, Jung SH, Bak JP, Lee SS, Rhee DK. Comparison of the rabbit pyrogen test and Limulus amoebocyte lysate (LAL) assay for endotoxin in hepatitis B vaccines and the effect of aluminum hydroxide. Biologicals. 2005; 33(3): 145-51[DOI][PubMed]
  • 22. Moldenhauer J, Sutton SV. Towards an improved sterility test. PDA J Pharm Sci Technol. 2004; 58(6): 284-6[PubMed]
  • 23. Gorska P. Principles in laboratory animal research for experimental purposes. Med Sci Monit. 2000; 6(1): 171-80[PubMed]
  • 24. Ferreira RB, Valdez Y, Coombes BK, Sad S, Gouw JW, Brown EM, et al. A Highly Effective Component Vaccine against Nontyphoidal Salmonella enterica Infections. MBio. 2015; 6(5): 1421-15[DOI][PubMed]
  • 25. De Ridder L, Maes D, Dewulf J, Butaye P, Pasmans F, Boyen F, et al. Use of a live attenuated Salmonella enterica serovar Typhimurium vaccine on farrow-to-finish pig farms. Vet J. 2014; 202(2): 303-8[DOI][PubMed]
  • 26. Periaswamy B, Maier L, Vishwakarma V, Slack E, Kremer M, Andrews-Polymenis HL, et al. Live attenuated S. Typhimurium vaccine with improved safety in immuno-compromised mice. PLoS One. 2012; 7(9): 45433[DOI][PubMed]
  • 27. Robinson A, Cranage MP, Hudson MJ. Vaccine protocols. 2003; 87[DOI]
  • 28. Weintraub A. Immunology of bacterial polysaccharide antigens. Carbohydr Res. 2003; 338(23): 2539-47[PubMed]
  • 29. Faezi S, Safarloo M, Amirmozafari N, Nikokar I, Siadat SD, Holder IA, et al. Protective efficacy of Pseudomonas aeruginosa type-A flagellin in the murine burn wound model of infection. APMIS. 2014; 122(2): 115-27[DOI][PubMed]
  • 30. Izgi K, Iskender B, Sakalar C, Arslanhan A, Saraymen B, Canatan H. Evaluation of two different adjuvants with immunogenic uroplakin 3A-derived peptide for their ability to evoke an immune response in mice. Eur Cytokine Netw. 2015; 26(2): 46-56[DOI][PubMed]
  • 31. Lanzilao L, Stefanetti G, Saul A, MacLennan CA, Micoli F, Rondini S. Strain Selection for Generation of O-Antigen-Based Glycoconjugate Vaccines against Invasive Nontyphoidal Salmonella Disease. PLoS One. 2015; 10(10): 139847[DOI][PubMed]
  • 32. Song L, Xiong D, Kang X, Yang Y, Wang J, Guo Y, et al. An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin. BMC Biotechnol. 2015; 15: 79[DOI][PubMed]
  • 33. Allam US, Krishna MG, Lahiri A, Joy O, Chakravortty D. Salmonella enterica serovar Typhimurium lacking hfq gene confers protective immunity against murine typhoid. PLoS One. 2011; 6(2): 16667[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments