Iranian Red Crescent Medical Journal

Published by: Kowsar

Protective Effects of Melissa officinalis (Lemon Balm) on Sperm Parameters and Spermatogenesis Quality in Rats Exposed to Lead

Mehran Mesgari Abbasi 1 , Narjeskhaton Dadkhah 2 , * , Mahnaz Shahnazi 3 and Neda Parvin 2
Authors Information
1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
2 Faculty Member of Nursing and Midwifery Department, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
3 Faculty Member of Nursing and Midwifery Department, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Iranian Red Crescent Medical Journal: December 01, 2016, 18 (12); e31798
  • Published Online: August 9, 2016
  • Article Type: Research Article
  • Received: July 25, 2015
  • Revised: December 8, 2015
  • Accepted: December 30, 2015
  • DOI: 10.5812/ircmj.31798

To Cite: Mesgari Abbasi M, Dadkhah N, Shahnazi M, Parvin N. Protective Effects of Melissa officinalis (Lemon Balm) on Sperm Parameters and Spermatogenesis Quality in Rats Exposed to Lead, Iran Red Crescent Med J. 2016 ; 18(12):e31798. doi: 10.5812/ircmj.31798.

Copyright © 2016, Iranian Red Crescent Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Ristanovic M, Bunjevacki V, Tulic C, Novakovic I, Nikolic A. Molecular analysis of Y chromosome microdeletions in idiopathic cases of male infertility in Serbia. Genetika. 2007; 43(6): 850-4[PubMed]
  • 2. Chowdhuri DK, Narayan R, Saxena DK. Effect of lead and chromium on nucleic acid and protein synthesis during sperm-zona binding in mice. Toxicol In Vitro. 2001; 15(6): 605-13[PubMed]
  • 3. Hu H, Shih R, Rothenberg S, Schwartz BS. The epidemiology of lead toxicity in adults: measuring dose and consideration of other methodologic issues. Environ Health Perspect. 2007; 115(3): 455-62[DOI][PubMed]
  • 4. Hayes CR, Skubala ND. Is there still a problem with lead in drinking water in the European Union? J Water Health. 2009; 7(4): 569-80[DOI][PubMed]
  • 5. Vincoli JW. Lead choloride. Risk management for hazardous chemicals. 1997; : 2005-10
  • 6. Ahrens FA. Effects of lead on glucose metabolism, ion flux, and collagen synthesis in cerebral capillaries of calves. Am J Vet Res. 1993; 54(5): 808-12[PubMed]
  • 7. Graca A, Ramalho-Santos J, de Lourdes Pereira M. Effect of lead chloride on spermatogenesis and sperm parameters in mice. Asian J Androl. 2004; 6(3): 237-41[PubMed]
  • 8. Farzadi L, Khaki A, Ouladsahebmadarek E, Ghadamkheir E, Khaki AA. Effect of rosmarinic acid on sexual behavior in diabetic male rats. African J Pharmacy Pharmacol. 2011; 5(16): 1906-10
  • 9. Anjum MR, Sainath SB, Suneetha Y, Reddy PS. Lead acetate induced reproductive and paternal mediated developmental toxicity in rats. Ecotoxicol Environ Saf. 2011; 74(4): 793-9[DOI][PubMed]
  • 10. Mohammadi M, Alipour M, Alipour MR, Vatankhah AM. Effects of high cholesterol diet and parallel chronic exercise on erythrocyte primary antioxidant enzymes and plasma total antioxidant capacity in Dutch rabbits. Int J Endocrinol Metab. 2006; 2006(1, Winter): 30-40
  • 11. Koca Y, Ozdal OL, Celik M, Unal S, Balaban N. Antioxidant activity of seminal plasma in fertile and infertile men. Arch Androl. 2003; 49(5): 355-9[PubMed]
  • 12. Sadraei H, Ghannadi A, Malekshahi K. Relaxant effect of essential oil of Melissa officinalis and citral on rat ileum contractions. Fitoterapia. 2003; 74(5): 445-52[PubMed]
  • 13. Nouri MAK, Fathiazad FAK. Study of effects of onion and ginger the spermatojenez in rats. 2009; 30(2): 53-8
  • 14. Dorostghoal M, Seyyednejad SM, Jabari A. Protective effects of Fumaria parviflora L. on lead-induced testicular toxicity in male rats. Andrologia. 2014; 46(4): 437-46[DOI][PubMed]
  • 15. Pryce JE, Royal MD, Garnsworthy PC, Mao IL. Fertility in the high-producing dairy cow. Livestock production science. 2004; 86(1): 125-35
  • 16. Dastmalchi K, Dorman HJD, Oinonen PP, Darwis Y, Laakso I, Hiltunen R. Chemical composition and in vitro antioxidative activity of a lemon balm (Melissa officinalis L.) extract. LWT Food Sci Technol. 2008; 41(3): 391-400
  • 17. Golshan IF, Emami E. The effects of lead on motility, viability and DNA denaturation of cauda epididymal spermatozoa of mouse. J Shahrekord Univ Med Sci. 2011; 13(4): 1-8
  • 18. Acharya UR, Mishra M, Patro J, Panda MK. Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium. Reprod Toxicol. 2008; 25(1): 84-8[DOI][PubMed]
  • 19. Tejada RI, Mitchell JC, Norman A, Marik JJ, Friedman S. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril. 1984; 42(1): 87-91[PubMed]
  • 20. Abdolahi M, Khordandi L, Ahrari K. The protective effect of green tea extract on acetaminophen induced nephro-toxicity in mice. Arak Med Univ J. 2010; 13(1): 90-6
  • 21. Khosari A, Hoseinzadeh A. Separate effects and combination exercises and aerobic exercises corcomin the vulnerability and mobility sperm form and hormons reproduction in rats infected with lead. 2012; : 22-3
  • 22. Narayana K, Prashanthi N, Nayanatara A, Kumar HH, Abhilash K, Bairy KL. Effects of methyl parathion (o,o-dimethyl o-4-nitrophenyl phosphorothioate) on rat sperm morphology and sperm count, but not fertility, are associated with decreased ascorbic acid level in the testis. Mutat Res. 2005; 588(1): 28-34[DOI][PubMed]
  • 23. Priya PH, Reddy PS. Effect of restraint stress on lead-induced male reproductive toxicity in rats. J Exp Zool A Ecol Genet Physiol. 2012; 317(7): 455-65[DOI][PubMed]
  • 24. Ibrahim NM, Eweis EA, El-Beltagi HS, Abdel-Mobdy YE. Retraction note to: The effect of lead acetate toxicity on experimental male albino rat. Biol Trace Elem Res. 2013; 151(1): 156[DOI][PubMed]
  • 25. Al-Attar AM. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J Biol Sci. 2011; 18(1): 63-72[DOI][PubMed]
  • 26. Chapin RE, Harris MW, Davis BJ, Ward SM, Wilson RE, Mauney MA, et al. The effects of perinatal/juvenile methoxychlor exposure on adult rat nervous, immune, and reproductive system function. Fundam Appl Toxicol. 1997; 40(1): 138-57[PubMed]
  • 27. Gruenwald J, Brendle JC. PDR for herbal medicine. 2000;
  • 28. Jalili A, Jamzad Z. Red data book of Iran: A preliminary survey of endemic, rare and endangered plant species in Iran. Research Institute of Forests and Rangelands. 1999; (1999-215)
  • 29. Murray MT. The healing power of herbs Rocklin. 1995; : 173-83
  • 30. Wright C, Milne S, Leeson H. Sperm DNA damage caused by oxidative stress: modifiable clinical, lifestyle and nutritional factors in male infertility. Reprod Biomed Online. 2014; 28(6): 684-703[DOI][PubMed]
  • 31. Aitken RJ, De Iuliis GN. On the possible origins of DNA damage in human spermatozoa. Mol Hum Reprod. 2010; 16(1): 3-13[DOI][PubMed]
  • 32. Koziorowska-Gilun M, Koziorowski M, Fraser L, Strzezek J. Antioxidant defence system of boar cauda epididymidal spermatozoa and reproductive tract fluids. Reprod Domest Anim. 2011; 46(3): 527-33[DOI][PubMed]
  • 33. Garg S, Doncel G, Chabra S, Upadhyay SN, Talwar GP. Synergistic spermicidal activity of neem seed extract, reetha saponins and quinine hydrochloride. Contraception. 1994; 50(2): 185-90[PubMed]
  • 34. Leiva KP, Rubio J, Peralta F, Gonzales GF. Effect of Punica granatum (pomegranate) on sperm production in male rats treated with lead acetate. Toxicol Mech Methods. 2011; 21(6): 495-502[DOI][PubMed]
  • 35. Cuerda C, Luengo LM, Valero MA, Vidal A, Burgos R, Calvo FL, et al. [Antioxidants and diabetes mellitus: review of the evidence]. Nutr Hosp. 2011; 26(1): 68-78[PubMed]
  • 36. Tagashira M, Ohtake Y. A new antioxidative 1, 3-benzodioxole from Melissa officinalis. Planta Medica. 1998; 64(6): 555-8
  • 37. Puerta Suarez J, Cardona Duque D, Alvarez Gomez A, Arango V, Cadavid A, Cardona Maya W. Efecto del extracto de Anethum graveolens, Melissa officinalis y Calendula officinalis sobre espermatozoides humanos. Revista Cubana de Plantas Medicinales. 2012; 17(4): 420-30
  • 38. Retana-Marquez S, Bonilla-Jaime H, Vazquez-Palacios G, Martinez-Garcia R, Velazquez-Moctezuma J. Changes in masculine sexual behavior, corticosterone and testosterone in response to acute and chronic stress in male rats. Horm Behav. 2003; 44(4): 327-37[PubMed]
  • 39. Collodel G, Moretti E, Fontani V, Rinaldi S, Aravagli L, Sarago G, et al. Effect of emotional stress on sperm quality. Indian J Med Res. 2008; 128(3): 254-61[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments