Iranian Red Crescent Medical Journal

Published by: Kowsar

Caloric Restriction and Formalin-Induced Inflammation: An Experimental Study in Rat Model

Aisan Nozad 1 , Mir Bahram Safari 2 , Ehsan Saboory 3 , Leila Derafshpoor 3 , Parvaneh Mohseni Moghaddam 4 , Farzaneh Ghaffari 5 and Mohsen Naseri 4 , *
Authors Information
1 Traditional Iranian Medicine Department, Medicine Faculty, Shahed University, Tehran, IR Iran
2 Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IR Iran
3 Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, IR Iran
4 Traditional Medicine Clinical Trial Research Center, Shahed University, Tehran, IR Iran
5 School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Article information
  • Iranian Red Crescent Medical Journal: July 01, 2015, 17 (7); e22590
  • Published Online: July 23, 2015
  • Article Type: Research Article
  • Received: August 6, 2014
  • Revised: September 6, 2014
  • Accepted: September 23, 2014
  • DOI: 10.5812/ircmj.22590v2

To Cite: Nozad A, Safari M B, Saboory E, Derafshpoor L, Mohseni Moghaddam P, et al. Caloric Restriction and Formalin-Induced Inflammation: An Experimental Study in Rat Model, Iran Red Crescent Med J. 2015 ; 17(7):e22590. doi: 10.5812/ircmj.22590v2.

Copyright © 2015, Iranian Red Crescent Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Nikolich-Zugich J, Messaoudi I. Mice and flies and monkeys too: caloric restriction rejuvenates the aging immune system of non-human primates. Exp Gerontol. 2005; 40(11): 884-93[DOI][PubMed]
  • 2. Tsuchiya T, Higami Y, Komatsu T, Tanaka K, Honda S, Yamaza H, et al. Acute stress response in calorie-restricted rats to lipopolysaccharide-induced inflammation. Mech Ageing Dev. 2005; 126(5): 568-79[DOI][PubMed]
  • 3. Longo VD, Finch CE. Evolutionary medicine: from dwarf model systems to healthy centenarians? Science. 2003; 299(5611): 1342-6[DOI][PubMed]
  • 4. Dixit VD, Yang H, Sayeed KS, Stote KS, Rumpler WV, Baer DJ, et al. Controlled meal frequency without caloric restriction alters peripheral blood mononuclear cell cytokine production. J Inflamm (Lond). 2011; 8: 6[DOI][PubMed]
  • 5. Ghods R, Gharooni M, Amin G, Nazem E, Nikbakht Nasrabadi A. Hypertension from the perspective of Iranian traditional medicine. Iran Red Crescent Med J. 2014; 16(3)[DOI][PubMed]
  • 6. Kim HJ, Jung KJ, Yu BP, Cho CG, Choi JS, Chung HY. Modulation of redox-sensitive transcription factors by calorie restriction during aging. Mech Ageing Dev. 2002; 123(12): 1589-95[PubMed]
  • 7. Matarese G, La Cava A. The intricate interface between immune system and metabolism. Trends Immunol. 2004; 25(4): 193-200[DOI][PubMed]
  • 8. Kumar VL, Basu N. Anti-inflammatory activity of the latex of Calotropis procera. J Ethnopharmacol. 1994; 44(2): 123-5[PubMed]
  • 9. Fereidoni M, Ahmadiani A, Semnanian S, Javan M. An accurate and simple method for measurement of paw edema. J Pharmacol Toxicol Methods. 2000; 43(1): 11-4[PubMed]
  • 10. Morgan TE, Wong AM, Finch CE. Anti-inflammatory mechanisms of dietary restriction in slowing aging processes. Interdiscip Top Gerontol. 2007; 35: 83-97[DOI][PubMed]
  • 11. Huang P, Li S, Shao M, Qi Q, Zhao F, You J, et al. Calorie restriction and endurance exercise share potent anti-inflammatory function in adipose tissues in ameliorating diet-induced obesity and insulin resistance in mice. Nutr Metab (Lond). 2010; 7: 59[DOI][PubMed]
  • 12. Heilbronn LK, de Jonge L, Frisard MI, DeLany JP, Larson-Meyer DE, Rood J, et al. Effect of 6-month calorie restriction on biomarkers of longevity, metabolic adaptation, and oxidative stress in overweight individuals: a randomized controlled trial. JAMA. 2006; 295(13): 1539-48[DOI][PubMed]
  • 13. Kirkwood TL, Kapahi P, Shanley DP. Evolution, stress, and longevity. J Anat. 2000; 197 Pt 4: 587-90[PubMed]
  • 14. Naseri M, Mojab F, Khodadoost M, Kamalinejad M, Davati A, Choopani R, et al. The Study of Anti-Inflammatory Activity of Oil-Based Dill (Anethum graveolens L.) Extract Used Topically in Formalin-Induced Inflammation Male Rat Paw. Iran J Pharm Res. 2012; 11(4): 1169-74[PubMed]
  • 15. Abbasian A, Naseri M, Masoud A. Anti-inflammatory effects of Urtica pilulifera l. seeds extracts in the rat. Iran J Pharm Res. 2010; 3(2): 49
  • 16. Soltanian A, Faghihzadeh S, Mehdibarzi D, Gerami A, Nasery M, Cheng J. Assessment of marhame-mafasel pomade effect on knee osteoarthritis with non-compliance. J Res Health Sci. 2009; 9(2): 19-24[PubMed]
  • 17. Fauci AS, Braunwald E, Kasper DL. Harrison's principles of internal medicine. 2012;
  • 18. Fouladi RF. Aqueous extract of dried fruit of Berberis vulgaris L. in acne vulgaris, a clinical trial. J Diet Suppl. 2012; 9(4): 253-61[DOI][PubMed]
  • 19. McCay CM, Maynard LA, Sperling G, Barnes LL. The Journal of Nutrition. Volume 18 July--December, 1939. Pages 1--13. Retarded growth, life span, ultimate body size and age changes in the albino rat after feeding diets restricted in calories. Nutr Rev. 1975; 33(8): 241-3[PubMed]
  • 20. Avicenna H. The Canon of Medicine. 2005;
  • 21. Nicklas BJ, Ambrosius W, Messier SP, Miller GD, Penninx BW, Loeser RF, et al. Diet-induced weight loss, exercise, and chronic inflammation in older, obese adults: a randomized controlled clinical trial. Am J Clin Nutr. 2004; 79(4): 544-51[PubMed]
  • 22. Nicklas BJ, You T, Pahor M. Behavioural treatments for chronic systemic inflammation: effects of dietary weight loss and exercise training. CMAJ. 2005; 172(9): 1199-209[DOI][PubMed]
  • 23. Martin B, Mattson MP, Maudsley S. Caloric restriction and intermittent fasting: two potential diets for successful brain aging. Ageing Res Rev. 2006; 5(3): 332-53[DOI][PubMed]
  • 24. Messaoudi I, Warner J, Fischer M, Park B, Hill B, Mattison J, et al. Delay of T cell senescence by caloric restriction in aged long-lived nonhuman primates. Proc Natl Acad Sci U S A. 2006; 103(51): 19448-53[DOI][PubMed]
  • 25. Zhou Q, Leeman SE, Amar S. Signaling mechanisms involved in altered function of macrophages from diet-induced obese mice affect immune responses. Proc Natl Acad Sci U S A. 2009; 106(26): 10740-5[DOI][PubMed]
  • 26. Wang J, Vanegas SM, Du X, Noble T, Zingg JM, Meydani M, et al. Caloric restriction favorably impacts metabolic and immune/inflammatory profiles in obese mice but curcumin/piperine consumption adds no further benefit. Nutr Metab (Lond). 2013; 10(1): 29[DOI][PubMed]
  • 27. Milner JJ, Beck MA. The impact of obesity on the immune response to infection. Proc Nutr Soc. 2012; 71(2): 298-306[DOI][PubMed]
  • 28. Smith JV, Heilbronn LK, Ravussin E. Energy restriction and aging. Curr Opin Clin Nutr Metab Care. 2004; 7(6): 615-22[PubMed]
  • 29. Heilbronn LK, Noakes M, Clifton PM. Energy restriction and weight loss on very-low-fat diets reduce C-reactive protein concentrations in obese, healthy women. Arterioscler Thromb Vasc Biol. 2001; 21(6): 968-70[PubMed]
  • 30. Ahmed T, Das SK, Golden JK, Saltzman E, Roberts SB, Meydani SN. Calorie restriction enhances T-cell-mediated immune response in adult overweight men and women. J Gerontol A Biol Sci Med Sci. 2009; 64(11): 1107-13[DOI][PubMed]
  • 31. Cefalu WT, Wagner JD, Wang ZQ, Bell-Farrow AD, Collins J, Haskell D, et al. A study of caloric restriction and cardiovascular aging in cynomolgus monkeys (Macaca fascicularis): a potential model for aging research. J Gerontol A Biol Sci Med Sci. 1997; 52(1)-9[PubMed]
  • 32. Flachs P, Rossmeisl M, Bryhn M, Kopecky J. Cellular and molecular effects of n-3 polyunsaturated fatty acids on adipose tissue biology and metabolism. Clin Sci (Lond). 2009; 116(1): 1-16[DOI][PubMed]
  • 33. Cao SX, Dhahbi JM, Mote PL, Spindler SR. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice. Proc Natl Acad Sci U S A. 2001; 98(19): 10630-5[DOI][PubMed]
  • 34. Yang H, Youm YH, Dixit VD. Inhibition of thymic adipogenesis by caloric restriction is coupled with reduction in age-related thymic involution. J Immunol. 2009; 183(5): 3040-52[DOI][PubMed]
  • 35. Kalinski P. Regulation of immune responses by prostaglandin E2. J Immunol. 2012; 188(1): 21-8[DOI][PubMed]
  • 36. Kim YJ, Kim HJ, No JK, Chung HY, Fernandes G. Anti-inflammatory action of dietary fish oil and calorie restriction. Life Sci. 2006; 78(21): 2523-32[DOI][PubMed]
  • 37. Dixit VD, Schaffer EM, Pyle RS, Collins GD, Sakthivel SK, Palaniappan R, et al. Ghrelin inhibits leptin- and activation-induced proinflammatory cytokine expression by human monocytes and T cells. J Clin Invest. 2004; 114(1): 57-66[DOI][PubMed]
  • 38. Ye J, Keller JN. Regulation of energy metabolism by inflammation: a feedback response in obesity and calorie restriction. Aging (Albany NY). 2010; 2(6): 361-8[PubMed]
  • 39. Gabriely I, Ma XH, Yang XM, Atzmon G, Rajala MW, Berg AH, et al. Removal of visceral fat prevents insulin resistance and glucose intolerance of aging: an adipokine-mediated process? Diabetes. 2002; 51(10): 2951-8[PubMed]
  • 40. Nguyen MT, Favelyukis S, Nguyen AK, Reichart D, Scott PA, Jenn A, et al. A subpopulation of macrophages infiltrates hypertrophic adipose tissue and is activated by free fatty acids via Toll-like receptors 2 and 4 and JNK-dependent pathways. J Biol Chem. 2007; 282(48): 35279-92[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments