Iranian Red Crescent Medical Journal

Published by: Kowsar

A Simple Co-culture System for Generation of Embryonic Stem-Like Cells From Testis

Maryam Nazm Bojnordi 1 , Mansoureh Movahedin 1 , * , Taki Tiraihi 1 and Mohamad Javan 2
Authors Information
1 Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, IR Iran
2 Department of Physiology, Medical Sciences Faculty, Tarbiat Modares University, Tehran, IR Iran
Article information
  • Iranian Red Crescent Medical Journal: December 6, 2012, 14 (12); 811-5
  • Published Online: December 6, 2012
  • Article Type: Research Article
  • Received: January 1, 2012
  • Revised: June 14, 2012
  • Accepted: July 6, 2012
  • DOI: 10.5812/ircmj.4051

To Cite: Nazm Bojnordi M, Movahedin M, Tiraihi T, Javan M. A Simple Co-culture System for Generation of Embryonic Stem-Like Cells From Testis, Iran Red Crescent Med J. 2012 ; 14(12):811-5. doi: 10.5812/ircmj.4051.

Copyright © 2012, Iranian Red Crescent Medical Journal. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Evans M, Hunter S. Source and nature of embryonic stem cells. C R Biol. 2002; 325(10): 1003-7[DOI]
  • 2. Rossant J. Stem cells from the Mammalian blastocyst. Stem Cells. 2001; 19(6): 477-82[DOI][PubMed]
  • 3. Turnpenny L, Cameron IT, Spalluto CM, Hanley KP, Wilson DI, Hanley NA. Human embryonic germ cells for future neuronal replacement therapy. Brain Res Bull. 2005; 6(1-2): 76-82[DOI][PubMed]
  • 4. Baba S, Heike T, Umeda K, Iwasa T, Kaichi S, Hiraumi Y, et al. Generation of cardiac and endothelial cells from neonatal mouse testis-derived multipotent germline stem cells. Stem Cells. 2007; 25(6): 1375-83[DOI][PubMed]
  • 5. Boulanger CA, Mack DL, Booth BW, Smith GH. Interaction with the mammary microenvironment redirects spermatogenic cell fate in vivo. Proc Natl Acad Sci U S A. 2007; 104(10): 3871-6[DOI][PubMed]
  • 6. de Rooij DG, Mizrak SC. Deriving multipotent stem cells from mouse spermatogonial stem cells: a new tool for developmental and clinical research. Development. 2008; 135(13): 2207-13[DOI][PubMed]
  • 7. Cyranoski D. Stem cells from testes: could it work? Nature. 2006; 440(7084): 586-7[DOI][PubMed]
  • 8. Guan K, Nayernia K, Maier LS, Wagner S, Dressel R, Lee JH, et al. Pluripotency of spermatogonial stem cells from adult mouse testis. Nature. 2006; 440(7088): 1199-203[DOI][PubMed]
  • 9. Kanatsu-Shinohara M, Lee J, Inoue K, Ogonuki N, Miki H, Toyokuni S, et al. Pluripotency of a single spermatogonial stem cell in mice. Biol Reprod. 2008; 78(4): 681-7[DOI][PubMed]
  • 10. Kanatsu-Shinohara M, Shinohara T. The germ of pluripotency. Nat Biotechnol. 2006; 24(6): 663-4[DOI][PubMed]
  • 11. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4): 663-76[DOI][PubMed]
  • 12. Nayernia K. Stem cells derived from testis show promise for treating a wide variety of medical conditions. Cell Res. 2007; 17(11): 895-7[DOI][PubMed]
  • 13. Nayernia K, Li M, Jaroszynski L, Khusainov R, Wulf G, Schwandt I, et al. Stem cell based therapeutical approach of male infertility by teratocarcinoma derived germ cells. Hum Mol Genet. 2004; 13(14): 1451-60[DOI][PubMed]
  • 14. Park IH, Arora N, Huo H, Maherali N, Ahfeldt T, Shimamura A, et al. Disease-specific induced pluripotent stem cells. Cell. 2008; 134(5): 877-86[DOI][PubMed]
  • 15. Takehashi M, Kanatsu-Shinohara M, Miki H, Lee J, Kazuki Y, Inoue K, et al. Production of knockout mice by gene targeting in multipotent germline stem cells. Dev Biol. 2007; 312(1): 344-52[DOI][PubMed]
  • 16. Guan K, Wagner S, Unsold B, Maier LS, Kaiser D, Hemmerlein B, et al. Generation of functional cardiomyocytes from adult mouse spermatogonial stem cells. Circ Res. 2007; 100(11): 1615-25[DOI][PubMed]
  • 17. Hofmann MC, Braydich-Stolle L, Dym M. Isolation of male germ-line stem cells; influence of GDNF. Dev Biol. 2005; 279(1): 114-24[DOI][PubMed]
  • 18. Kanatsu-Shinohara M, Inoue K, Lee J, Yoshimoto M, Ogonuki N, Miki H, et al. Generation of pluripotent stem cells from neonatal mouse testis. Cell. 2004; 119(7): 1001-12[DOI][PubMed]
  • 19. Seandel M, James D, Shmelkov SV, Falciatori I, Kim J, Chavala S, et al. Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature. 2007; 449(7160): 346-50[DOI][PubMed]
  • 20. Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, et al. Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod. 2003; 69(2): 612-6[DOI][PubMed]
  • 21. Kubota H, Brinster RL. Technology insight: In vitro culture of spermatogonial stem cells and their potential therapeutic uses. Nat Clin Pract Endocrinol Metab. 2006; 2(2): 99-108[DOI][PubMed]
  • 22. Mardanpour P, Guan K, Nolte J, Lee JH, Hasenfuss G, Engel W, et al. Potency of germ cells and its relevance for regenerative medicine. J Anat. 2008; 213(1): 26-9[DOI][PubMed]
  • 23. Kossack N, Meneses J, Shefi S, Nguyen HN, Chavez S, Nicholas C, et al. Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells. Stem Cells. 2009; 27(1): 138-49[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments