Transcriptional Expression of Bcl-2, Her2, VEGF, and hTERT in Caki-1 Human Renal Cancer Cells Modulated by Cornus mas Extract

Ji, Juan; Hou, Shufen; Gao, Yan; Feng, Xinli; Li, Song

doi:10.32592/ircmj.2020.22.11.198

Document Type : Research articles

Authors

¹ Nephrology department, Affiliated hospital of Hebei University, Baoding, Hebei, 071000, China.

² Nephrology department, Affiliated hospital of Hebei University, Baoding, Hebei, 071000, China

³ Department of Hyperbaric oxygen, Affiliated hospital of Hebei University, Baoding, Hebei, 071000, China.

⁴ Department of urinary surgery, Affiliated hospital of Hebei University, Baoding, Hebei, 071000, China

https://doi.org/10.32592/ircmj.2020.22.11.198

Abstract

Background: Herbal medicines, particularly those rich in polyphenolic compounds, have been proposed to be chemotherapeutic factors, which can modulate several pathways associated with cancer. To gain mechanistic insights into the anti-proliferative impacts of Cornus mas extract (CME), this study investigated the expression changes of several prominent genes, which involved in malignancy with therapeutic potential.
Objectives: The aim of the study was to determine the anticancer potential of CME on the main regulatory genes in renal carcinogenesis.
Methods: To perform the research, Caki-1 cancer cells were incubated for 72 h with 250 µg/ml of CME upon the cells with ribonucleic acids (RNAs) extracted for identified alterations of human telomerase reverse transcriptase (hTERT), vascular endothelial growth factor (VEGF), human epidermal growth factor receptor 2 (Her2), and B-cell lymphoma-2 (Bcl-2) gene expressions by a quantitative reverse transcription-polymerase chain reaction. The changes in protein expression were analyzed by the western blot method. Cell apoptosis was detected using the flow cytometry technique.
Results: Cornus mas extract caused down-regulated Bcl-2 as an anti-apoptotic 4.34-fold gene expression. Moreover, Her2 oncogene messenger RNA expression was inhibited by 250 µg/ml concentration of ~10-fold CME. The antitumor activity of CME was pronounced in its potent anti-angiogenic potential, as CME resulted in a striking decrease in ~125-fold expression of VEGF compared to the untreated control. In contrast, CME led to ~2.6-fold up-regulation of hTERT in Caki-1 cancer cells.
Conclusion: Overall, various molecular pathways were formed to interplay with Caki-1 cells, which depended on the active phenolic compound of CME. It is recommended to perform further studies to investigate the effect of unique polyphenols of the total extract of CME to establish an effective strategy for renal cancer treatment.

Keywords

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Iranian Red Crescent Medical Journal (IRCMJ)

Transcriptional Expression of Bcl-2, Her2, VEGF, and hTERT in Caki-1 Human Renal Cancer Cells Modulated by Cornus mas Extract

References

References

Volume 22, Issue 11 - Serial Number 11
2020

Transcriptional Expression of Bcl-2, Her2, VEGF, and hTERT in Caki-1 Human Renal Cancer Cells Modulated by Cornus mas Extract

References

References

Volume 22, Issue 11 - Serial Number 11 2020

Volume 22, Issue 11 - Serial Number 11
2020