Iranian Red Crescent Medical Journal Iranian Red Crescent Medical Journal Iran Red Crescent Med J http://www.ircmj.com 2074-1804 2074-1812 10.5812/ircmj en jalali 2017 6 28 gregorian 2017 6 28 14 9
en Late Development of Pancreatitis Following Gunshot Trauma, A Case Report Late Development of Pancreatitis Following Gunshot Trauma, A Case Report case-report case-report Background

Gunshot trauma to the pancreatic duct mainly have been published from wartime experiences, but bullet injury in these cases has lead to pancreatic duct disruption, not obstruction. We report a case of chronic pancreatitis which is presented 27 years following pancreatic duct obstruction due to bullet injury during wartime, which successfully treated. He was a 45-year-old man came with chronic epigastric abdominal pain. Physical examination was suggestive of pancreatitis and laboratory data confirmed the diagnosis. Imaging studies revealed a metallic object in main pancreatic duct. He carefully treated with pancreatic head resection and pancreaticojejunal anastomosis.

Background

Gunshot trauma to the pancreatic duct mainly have been published from wartime experiences, but bullet injury in these cases has lead to pancreatic duct disruption, not obstruction. We report a case of chronic pancreatitis which is presented 27 years following pancreatic duct obstruction due to bullet injury during wartime, which successfully treated. He was a 45-year-old man came with chronic epigastric abdominal pain. Physical examination was suggestive of pancreatitis and laboratory data confirmed the diagnosis. Imaging studies revealed a metallic object in main pancreatic duct. He carefully treated with pancreatic head resection and pancreaticojejunal anastomosis.

Late;Pancreatitis;Gunshot;Trauma Late;Pancreatitis;Gunshot;Trauma 584 586 http://www.ircmj.com/index.php?page=article&article_id=3629 S Nikeghbalian S Nikeghbalian Associate Professor of Surgery, Shiraz University of Medical Sciences, Iran Associate Professor of Surgery, Shiraz University of Medical Sciences, Iran M Akrami M Akrami Ge~erumeurogoomnwzi~a{uwomv}~s{|yhof Oegikim [o{uowe, Zand Blv., Iran +98 7112330724, makrami@sums.ac.ir; Ge~erumeurogoomnwzi~a{uwomv}~s{|yhof Oegikim [o{uowe, Zand Blv., Iran +98 7112330724, makrami@sums.ac.ir Ge~erumeurogoomnwzi~a{uwomv}~s{|yhof Oegikim [o{uowe, Zand Blv., Iran +98 7112330724, makrami@sums.ac.ir; Ge~erumeurogoomnwzi~a{uwomv}~s{|yhof Oegikim [o{uowe, Zand Blv., Iran +98 7112330724, makrami@sums.ac.ir A Fazelzadeh A Fazelzadeh Resident of General Surgery, Shiraz University of Medical sciences, Iran Resident of General Surgery, Shiraz University of Medical sciences, Iran
en Establishment of a standard seed lot system of an Iranian Mumps virus strain; RS-12, for mass production of mumps and MMR vaccines Establishment of a standard seed lot system of an Iranian Mumps virus strain; RS-12, for mass production of mumps and MMR vaccines research-article research-article Background

At present the mumps virus strain used for production of mumps vaccine for our local use is Hoshino strain. However, according to our National public health policies, this strain should be replaced with a safer strain. Based on our previous data, the Iranian mumps strain; RS-12 has been proved to be the most suitable alternative to Hoshino strain with little or no adverse events following vaccination

Methods

The aim of the present study was to optimize propagation of RS-12 strain and prepare standard seeds for vaccine mass production. The virus was inoculated to cells using different methods and different multiplicity of infection (MOI). The viral suspensions were harvested using different methods. Quality control tests were run at different stages.

Results

Maximum viral yield was achieved when cell suspensions were inoculated at MOI of 1:10 and incubated at 36-37ºC for 48 hours, followed by replacement of the media and incubation at 33-34 ºC for 5-7 days. Filtration did not affect the viral titre. A standard seed lot system was successfully established and experimental batches of MMR vaccines were produced.

Conclusion

The established seed lot system has met all requirements of WHO regulations and could be used in mass production of safe and efficacious mumps and MMR vaccines. Clinical trials are in progress for this newly produced vaccine.

Background

At present the mumps virus strain used for production of mumps vaccine for our local use is Hoshino strain. However, according to our National public health policies, this strain should be replaced with a safer strain. Based on our previous data, the Iranian mumps strain; RS-12 has been proved to be the most suitable alternative to Hoshino strain with little or no adverse events following vaccination

Methods

The aim of the present study was to optimize propagation of RS-12 strain and prepare standard seeds for vaccine mass production. The virus was inoculated to cells using different methods and different multiplicity of infection (MOI). The viral suspensions were harvested using different methods. Quality control tests were run at different stages.

Results

Maximum viral yield was achieved when cell suspensions were inoculated at MOI of 1:10 and incubated at 36-37ºC for 48 hours, followed by replacement of the media and incubation at 33-34 ºC for 5-7 days. Filtration did not affect the viral titre. A standard seed lot system was successfully established and experimental batches of MMR vaccines were produced.

Conclusion

The established seed lot system has met all requirements of WHO regulations and could be used in mass production of safe and efficacious mumps and MMR vaccines. Clinical trials are in progress for this newly produced vaccine.

RS-12;MMR;mumps;vaccine RS-12;MMR;mumps;vaccine 508 514 http://www.ircmj.com/index.php?page=article&article_id=913 M K Shahkarami M K Shahkarami Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran T Mokhtari Azad T Mokhtari Azad Head of Virology Department, School of Public Health, Tehran University of Medical Sciences, Iran ; Head of Virology Department, School of Public Health, Tehran University of Medical Sciences, Iran Head of Virology Department, School of Public Health, Tehran University of Medical Sciences, Iran ; Head of Virology Department, School of Public Health, Tehran University of Medical Sciences, Iran K Aghaiypour K Aghaiypour Head of Genomics Lab., Biotechnology Department, Razi Vaccine and Serum Research Institute, Iran Head of Genomics Lab., Biotechnology Department, Razi Vaccine and Serum Research Institute, Iran A Shafyi A Shafyi Member of scientific board, Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran Member of scientific board, Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran M Taqavian M Taqavian Member of scientific board, Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran Member of scientific board, Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran A Mohammadi A Mohammadi Head of Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran Head of Human Viral Vaccines Department, Razi Vaccine and Serum Research Institute, Iran
en The Efficacy of Percutaneous Vertebroplasty in Pain Relief in Patients with Pathological Vertebral Fractures due to Metastatic Spinal Tumors The Efficacy of Percutaneous Vertebroplasty in Pain Relief in Patients with Pathological Vertebral Fractures due to Metastatic Spinal Tumors research-article research-article Background

Metastatic spinal tumors are common and major causes of pathological spinal fractures that result in severe pain, weakness, and progressive neurological deficits. This study aims to evaluate the efficacy of percutaneous vertebroplasty (PVP) in pain-relief in patients with spinal fractures due to metastatic spinal tumors.

Methods

We evaluated 25 documented cases of metastatic spinal tumors with pathologic vertebral fractures who were suffering from severe pain and underwent vertebroplasty. Degree of pain was measured by visual analog scale (VAS). The symptoms were evaluated 24 hours and 2 months after vertebroplasty regarding the degree of pain relief.Complications such as leakage, embolism and infection were assessed.

Results

MeanVAS score was 8.23 before therapy in the patients that was reduced to 2.12 and 1 in the patients 24 hours and 2 months after vertebroplasty, respectively. The most common complication was cement leakage (44%) and there was no embolism or infection. Data was analyzed by SPSS version 18 software through ANOVA test with Greenhouse-Geisser correction and P-value of 0.00 was obtained in the patients 24 hours and 1 month after surgery.

Conclusion

Considering significant decrease in the mean pain severity degree after the treatment, veretebroplasty seems to be significantly effective in pain relief in metastatic spinal tumors.

Background

Metastatic spinal tumors are common and major causes of pathological spinal fractures that result in severe pain, weakness, and progressive neurological deficits. This study aims to evaluate the efficacy of percutaneous vertebroplasty (PVP) in pain-relief in patients with spinal fractures due to metastatic spinal tumors.

Methods

We evaluated 25 documented cases of metastatic spinal tumors with pathologic vertebral fractures who were suffering from severe pain and underwent vertebroplasty. Degree of pain was measured by visual analog scale (VAS). The symptoms were evaluated 24 hours and 2 months after vertebroplasty regarding the degree of pain relief.Complications such as leakage, embolism and infection were assessed.

Results

MeanVAS score was 8.23 before therapy in the patients that was reduced to 2.12 and 1 in the patients 24 hours and 2 months after vertebroplasty, respectively. The most common complication was cement leakage (44%) and there was no embolism or infection. Data was analyzed by SPSS version 18 software through ANOVA test with Greenhouse-Geisser correction and P-value of 0.00 was obtained in the patients 24 hours and 1 month after surgery.

Conclusion

Considering significant decrease in the mean pain severity degree after the treatment, veretebroplasty seems to be significantly effective in pain relief in metastatic spinal tumors.

Polymethylmethacrylate (PMMA);Vertebroplasty;Spinal metastasis;Tumors;Spine;Pain Polymethylmethacrylate (PMMA);Vertebroplasty;Spinal metastasis;Tumors;Spine;Pain 523 530 http://www.ircmj.com/index.php?page=article&article_id=1288 Mr Farrokhi Mr Farrokhi S{{c{a~eurgmwowsoveeuro{usgov}~,skismzane}sk}enoeshsmseorcouoe~uwv, Department of Neurosurgery, Shiraz University of Medical Sciences, Iran. +98 -711-6234508, farokhim@sums.ac.ir; S{{c{a~eurgmwowsoveeuro{usgov}~,skismzane}sk}enoeshsmseorcouoe~uwv, Department of Neurosurgery, Shiraz University of Medical Sciences, Iran. +98 -711-6234508, farokhim@sums.ac.ir S{{c{a~eurgmwowsoveeuro{usgov}~,skismzane}sk}enoeshsmseorcouoe~uwv, Department of Neurosurgery, Shiraz University of Medical Sciences, Iran. +98 -711-6234508, farokhim@sums.ac.ir; S{{c{a~eurgmwowsoveeuro{usgov}~,skismzane}sk}enoeshsmseorcouoe~uwv, Department of Neurosurgery, Shiraz University of Medical Sciences, Iran. +98 -711-6234508, farokhim@sums.ac.ir H Nouraei H Nouraei Associate Professor of Orthopaedics, Shiraz Neurosciences Research Center, Department of Orthopaedic surgery, Shiraz University of Medical Sciences, Iran. Associate Professor of Orthopaedics, Shiraz Neurosciences Research Center, Department of Orthopaedic surgery, Shiraz University of Medical Sciences, Iran. A Kiani A Kiani Shiraz Neurosciences Research Center, Shiraz University of Medical Sciences, Iran. Shiraz Neurosciences Research Center, Shiraz University of Medical Sciences, Iran.
en Efficacy of omega-3 fatty acids supplementation in treatment of uremic pruritus in hemodialysis patients: a double-blind randomized controlled trial Efficacy of omega-3 fatty acids supplementation in treatment of uremic pruritus in hemodialysis patients: a double-blind randomized controlled trial research-article research-article Background

Uremic pruritus is a common and bothersome complaint among end-stage renal disease which affect between 25% and 60% of this population .But there is no decisive cure for treatment of it. In this study, the effects of omega-3 for treatment of pruritus were investigated in hemodialysis patients.

Methods

A double-blind randomized study was carried out in the form of placebo-controlled crossover study in four dialysis centers in Tehran, Iran during 2008. At first, 22 hemodialysis patients suffering from pruritus with previous drug resistance were selected. Next, these patients were randomly allocated into two groups of omega-3-placebo (group A) and placebo-omega-3 (group B) .Patients in group A were treated with a 1-gram Fish oil capsule for 20 days, and subsequently, they were treated with placebo for 20 days after a 14-day wash-out period .But the reverse act was done in group B.The pruritus assessment was made quantitatively through Detailed Pruritus Score.

Results

Pruritus was decreased up to 65% from score mean of 20.3 (95% CI: 16.7-23.8) to 6.4 (95% CI: 2.9-9.8) in omega-3 group and the decrease in the placebo group was 15% from score mean of 17.0 (95% CI: 12.4-21.6) to 14.4 (95% CI: 10.5-18.2).So the level of statistical difference was significant (P=0.0001).

Discussion

Omega-3 fatty acids found to be more effective than placebo in decreasing of uremic pruritus. So it seems that Omega-3 fatty acids could be used as an efficient drug for treatment of pruritus in uremic patients.

Background

Uremic pruritus is a common and bothersome complaint among end-stage renal disease which affect between 25% and 60% of this population .But there is no decisive cure for treatment of it. In this study, the effects of omega-3 for treatment of pruritus were investigated in hemodialysis patients.

Methods

A double-blind randomized study was carried out in the form of placebo-controlled crossover study in four dialysis centers in Tehran, Iran during 2008. At first, 22 hemodialysis patients suffering from pruritus with previous drug resistance were selected. Next, these patients were randomly allocated into two groups of omega-3-placebo (group A) and placebo-omega-3 (group B) .Patients in group A were treated with a 1-gram Fish oil capsule for 20 days, and subsequently, they were treated with placebo for 20 days after a 14-day wash-out period .But the reverse act was done in group B.The pruritus assessment was made quantitatively through Detailed Pruritus Score.

Results

Pruritus was decreased up to 65% from score mean of 20.3 (95% CI: 16.7-23.8) to 6.4 (95% CI: 2.9-9.8) in omega-3 group and the decrease in the placebo group was 15% from score mean of 17.0 (95% CI: 12.4-21.6) to 14.4 (95% CI: 10.5-18.2).So the level of statistical difference was significant (P=0.0001).

Discussion

Omega-3 fatty acids found to be more effective than placebo in decreasing of uremic pruritus. So it seems that Omega-3 fatty acids could be used as an efficient drug for treatment of pruritus in uremic patients.

Fatty Acids;Omega-3;Fish Oils;pruritus;Renal Dialysis;Uremia Fatty Acids;Omega-3;Fish Oils;pruritus;Renal Dialysis;Uremia 515 522 http://www.ircmj.com/index.php?page=article&article_id=1207 Esmat Ghanei Esmat Ghanei Usktoo~1psofgvaof9~mp{volowyduronogeand Nephrology Research Center(UNRC), Shohada Medical Center, Nephrology Dept., Shahid Beheshti University, M.C.(SBMU), I.R. Iran +98 21 22567222, dr_e_ghanei@yahoo.com; Usktoo~1psofgvaof9~mp{volowyduronogeand Nephrology Research Center(UNRC), Shohada Medical Center, Nephrology Dept., Shahid Beheshti University, M.C.(SBMU), I.R. Iran +98 21 22567222, dr_e_ghanei@yahoo.com Usktoo~1psofgvaof9~mp{volowyduronogeand Nephrology Research Center(UNRC), Shohada Medical Center, Nephrology Dept., Shahid Beheshti University, M.C.(SBMU), I.R. Iran +98 21 22567222, dr_e_ghanei@yahoo.com; Usktoo~1psofgvaof9~mp{volowyduronogeand Nephrology Research Center(UNRC), Shohada Medical Center, Nephrology Dept., Shahid Beheshti University, M.C.(SBMU), I.R. Iran +98 21 22567222, dr_e_ghanei@yahoo.com J Zeinali J Zeinali Resident of Internal Medicine, Shohada Medical Center, Internal Medicine Dept., SBMU, I.R. Iran Resident of Internal Medicine, Shohada Medical Center, Internal Medicine Dept., SBMU, I.R. Iran M Borghei M Borghei General Practitioner, UNRC, I.R. Iran General Practitioner, UNRC, I.R. Iran M Homayouni M Homayouni Assistant professor of Internal medicine, Department of Internal medicine, Shohada Hospital,Shahid Beheshti university of medical sciences. Assistant professor of Internal medicine, Department of Internal medicine, Shohada Hospital,Shahid Beheshti university of medical sciences.
en Burden and Typing of Rotavirus Group A in Children with Acute Gastroenteritis in Shiraz, Southern Iran Burden and Typing of Rotavirus Group A in Children with Acute Gastroenteritis in Shiraz, Southern Iran research-article research-article Background

Human Rotavirus is a significant cause of severe gastroenteritis in infants and young children worldwide. In recent years, Rotavirus genotyping by RT-PCR has provided valuable information about the diversity of Rotaviruses circulating worldwide. The purpose of the present study is to monitor the prevalence of the different G types of Rotaviruses circulating in Shiraz, Southern Iran and detect any uncommon or novel types.

Methods

During the period from December 2007 to November 2008, a total of 138 stool samples were collected from children less than 5 years old who were hospitalized for acute gastroenteritis. Rotavirus-associated diarrhea was investigated in fecal specimens with enzyme immunoassays (EIA). Rotavirus-positive specimens were typed by the Nested RT-PCR and by using different types of specific primers.

Results

Out of the 138 collected samples, 34.78% (48 cases) tested positive for Rotavirus. The frequency of G1, G2 and G4 types was 6.25%, 2.08% and 27.08%, respectively. Mixed and non-typeable infections were detected in 33.34% and 31.25% of hospitalized children with acute diarrhea, respectively. This is the first time mixed Rotavirus infections with G1/G3 have been reported in Iran.

Conclusion

The high frequency of Rotavirus detection indicates the severity and the burden of Rotavirus disease may be able to reduce through the implementation of an effective vaccine and continual surveillance for the detection of Rotavirus genotypes circulating in other regions of Iran. Regarding to the noticeable frequency of non-typeable and mixed infections, it is suggested to use the other specific primers and further studies to detection of other novel and unusual types.

Background

Human Rotavirus is a significant cause of severe gastroenteritis in infants and young children worldwide. In recent years, Rotavirus genotyping by RT-PCR has provided valuable information about the diversity of Rotaviruses circulating worldwide. The purpose of the present study is to monitor the prevalence of the different G types of Rotaviruses circulating in Shiraz, Southern Iran and detect any uncommon or novel types.

Methods

During the period from December 2007 to November 2008, a total of 138 stool samples were collected from children less than 5 years old who were hospitalized for acute gastroenteritis. Rotavirus-associated diarrhea was investigated in fecal specimens with enzyme immunoassays (EIA). Rotavirus-positive specimens were typed by the Nested RT-PCR and by using different types of specific primers.

Results

Out of the 138 collected samples, 34.78% (48 cases) tested positive for Rotavirus. The frequency of G1, G2 and G4 types was 6.25%, 2.08% and 27.08%, respectively. Mixed and non-typeable infections were detected in 33.34% and 31.25% of hospitalized children with acute diarrhea, respectively. This is the first time mixed Rotavirus infections with G1/G3 have been reported in Iran.

Conclusion

The high frequency of Rotavirus detection indicates the severity and the burden of Rotavirus disease may be able to reduce through the implementation of an effective vaccine and continual surveillance for the detection of Rotavirus genotypes circulating in other regions of Iran. Regarding to the noticeable frequency of non-typeable and mixed infections, it is suggested to use the other specific primers and further studies to detection of other novel and unusual types.

Human Rotavirus; Diarrhea; Hospitalized children; G types Human Rotavirus; Diarrhea; Hospitalized children; G types 531 540 http://www.ircmj.com/index.php?page=article&article_id=2082 A Najafi A Najafi Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com M Kargar M Kargar Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com; Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com; Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com T Jafarpour T Jafarpour Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com Ewsos}muotpofm{sbooloo{~ojio~ooynreosi~tmwnumog M{cvowoo~ow{mt{ahrom Branch, Islamic Azad University, IR Iran +98 917 3149203, microkargar@gmail.com
en Respiratory disorders associated with heavy inhalation exposure to dolomite dust Respiratory disorders associated with heavy inhalation exposure to dolomite dust research-article research-article Background

Although dolomite is classified as a relatively non-toxic, nuisance dust, little information exists as to its potential to produce respiratory disorders following occupational exposure. The purpose of this study was, therefore, to evaluate the possible effects, if any, of heavy inhalation exposure to this chemical on the prevalence of respiratory symptoms, functional impairments and radiographic abnormalities of the lungs.

Methods

The study population consisted of a group of 39 exposed subjects engaged in digging and excavating activities that were in operation for building a local dam, as well as 40 healthy non-exposed employees that served as the referent group. Subjects were interviewed and respiratory symptoms questionnaires, as suggested by the American Thoracic Society (ATS), were completed for them. Thereafter, they underwent chest X-ray and lung function tests. Additionally, using routine gravimetric techniques, personal dust monitoring for airborne inhalable and respirable dust was carried out at different dusty work sites. Finally to determine the chemical composition of the dust, it was analyzed by X-ray fluorescence (XRF) technique.

Results

XRF revealed that the major component (50.52%) of the dust was calcium magnesium carbonate, dolomite. Additionally, levels of exposure to inhalable and respirable dust were estimated to be 51.7±24.31 and 23.0±18.11mg/m3, respectively. Statistical analysis of the data showed that symptoms such as regular cough, phlegm, wheezing, productive cough and shortness of breath were significantly (p<0.05) more prevalent among exposed workers. Similarly, the ratio of FEV1/FVC in exposed subjects was significantly different from that of non-exposed individuals. In contrast, no significant abnormalities were observed in the chest radiographs of both groups.

Conclusions

In conclusion, while these data cast doubt on the notion that dolomite is a harmless chemical, they provide evidence in favour of the proposition that exposure to high atmospheric concentrations of this compound is likely to be associated with respiratory symptoms.

Background

Although dolomite is classified as a relatively non-toxic, nuisance dust, little information exists as to its potential to produce respiratory disorders following occupational exposure. The purpose of this study was, therefore, to evaluate the possible effects, if any, of heavy inhalation exposure to this chemical on the prevalence of respiratory symptoms, functional impairments and radiographic abnormalities of the lungs.

Methods

The study population consisted of a group of 39 exposed subjects engaged in digging and excavating activities that were in operation for building a local dam, as well as 40 healthy non-exposed employees that served as the referent group. Subjects were interviewed and respiratory symptoms questionnaires, as suggested by the American Thoracic Society (ATS), were completed for them. Thereafter, they underwent chest X-ray and lung function tests. Additionally, using routine gravimetric techniques, personal dust monitoring for airborne inhalable and respirable dust was carried out at different dusty work sites. Finally to determine the chemical composition of the dust, it was analyzed by X-ray fluorescence (XRF) technique.

Results

XRF revealed that the major component (50.52%) of the dust was calcium magnesium carbonate, dolomite. Additionally, levels of exposure to inhalable and respirable dust were estimated to be 51.7±24.31 and 23.0±18.11mg/m3, respectively. Statistical analysis of the data showed that symptoms such as regular cough, phlegm, wheezing, productive cough and shortness of breath were significantly (p<0.05) more prevalent among exposed workers. Similarly, the ratio of FEV1/FVC in exposed subjects was significantly different from that of non-exposed individuals. In contrast, no significant abnormalities were observed in the chest radiographs of both groups.

Conclusions

In conclusion, while these data cast doubt on the notion that dolomite is a harmless chemical, they provide evidence in favour of the proposition that exposure to high atmospheric concentrations of this compound is likely to be associated with respiratory symptoms.

Dolomite dust;Occupational exposure;Respiratory symptoms;Functional impairments of the lungs Dolomite dust;Occupational exposure;Respiratory symptoms;Functional impairments of the lungs 549 557 http://www.ircmj.com/index.php?page=article&article_id=2275 M Neghab M Neghab PhD, _sowuswonon Ogc}titmo~a~tmeo||hc{ooooofoneimultinennu~w}viooondszmse{rwniwe~wmvyfov Meemwilsskmoogw Shiraz University of Medical Sciences, Iran +98-711-7251020, neghabm@sums.ac.ir; PhD, _sowuswonon Ogc}titmo~a~tmeo||hc{ooooofoneimultinennu~w}viooondszmse{rwniwe~wmvyfov Meemwilsskmoogw Shiraz University of Medical Sciences, Iran +98-711-7251020, neghabm@sums.ac.ir PhD, _sowuswonon Ogc}titmo~a~tmeo||hc{ooooofoneimultinennu~w}viooondszmse{rwniwe~wmvyfov Meemwilsskmoogw Shiraz University of Medical Sciences, Iran +98-711-7251020, neghabm@sums.ac.ir; PhD, _sowuswonon Ogc}titmo~a~tmeo||hc{ooooofoneimultinennu~w}viooondszmse{rwniwe~wmvyfov Meemwilsskmoogw Shiraz University of Medical Sciences, Iran +98-711-7251020, neghabm@sums.ac.ir R Abedini R Abedini MSc Student, Occupational Health Department, School of Health and Nutrition, Shiraz University of Medical Sciences, Iran MSc Student, Occupational Health Department, School of Health and Nutrition, Shiraz University of Medical Sciences, Iran A Soltanzadeh A Soltanzadeh MSc, Occupational Health Department, School of Health and Nutrition, Shiraz University of Medical Sciences, Iran MSc, Occupational Health Department, School of Health and Nutrition, Shiraz University of Medical Sciences, Iran A Iloon Kashkooli A Iloon Kashkooli Former MPh student, School of Health and Nutrition, Shiraz University of Medical Sciences, Iran Former MPh student, School of Health and Nutrition, Shiraz University of Medical Sciences, Iran SMA Ghayoomi SMA Ghayoomi Pneumologist, Associate professor, Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Iran. Pneumologist, Associate professor, Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Iran.
en The diagnostic power of cervico-vaginal fluid prolactin in the diagnosis of premature rupture of membranes The diagnostic power of cervico-vaginal fluid prolactin in the diagnosis of premature rupture of membranes research-article research-article Background

Premature rupture of membranes (PROM) is one of the most common complications of pregnancy that has a major impact on pregnancy outcomes. A diagnostic tool that is non-invasive, specific and quick is needed to predict PROM. The purpose of this study was to evaluate the diagnostic power of the vaginal washing fluid prolactin for the diagnosis of premature rupture of membranes and to determine cut-off values.

Methods

A total of 114 pregnant women were recruited in this diagnostic trial. The PROM group consisted of 54 pregnant women between 20 and 41 weeks of gestation with diagnosis of confirmed PROM [amniotic fluid pooling (+) and Nitrazine paper test (+) and fernt test (+)]. The control group consisted of 60 pregnant women between 20 and 41 weeks of gestation without any complaint or complication. All patients underwent speculum examination for amniotic fluid pooling, nitrazine paper test, fern test, vaginal washing fluid prolactin sampling.

Results

Vaginal fluid concentrations of prolactin was significantly different between the two groups (P < 0.001). The sensitivity, specificity, positive and negative predictive values and accuracy were 87.03%, 75.0%, 75.80%, 86.53% and 83.33% in detecting PROM by evaluation of vaginal fluid prolactin concentration with a cut-off value of 9.50 µIU/ml, respectively.

Conclusions

The prolactin levels in the washing fluid of the posterior vaginal fornix in our experience is reliable and non-invasive diagnostic tests of PROM.

Background

Premature rupture of membranes (PROM) is one of the most common complications of pregnancy that has a major impact on pregnancy outcomes. A diagnostic tool that is non-invasive, specific and quick is needed to predict PROM. The purpose of this study was to evaluate the diagnostic power of the vaginal washing fluid prolactin for the diagnosis of premature rupture of membranes and to determine cut-off values.

Methods

A total of 114 pregnant women were recruited in this diagnostic trial. The PROM group consisted of 54 pregnant women between 20 and 41 weeks of gestation with diagnosis of confirmed PROM [amniotic fluid pooling (+) and Nitrazine paper test (+) and fernt test (+)]. The control group consisted of 60 pregnant women between 20 and 41 weeks of gestation without any complaint or complication. All patients underwent speculum examination for amniotic fluid pooling, nitrazine paper test, fern test, vaginal washing fluid prolactin sampling.

Results

Vaginal fluid concentrations of prolactin was significantly different between the two groups (P < 0.001). The sensitivity, specificity, positive and negative predictive values and accuracy were 87.03%, 75.0%, 75.80%, 86.53% and 83.33% in detecting PROM by evaluation of vaginal fluid prolactin concentration with a cut-off value of 9.50 µIU/ml, respectively.

Conclusions

The prolactin levels in the washing fluid of the posterior vaginal fornix in our experience is reliable and non-invasive diagnostic tests of PROM.

Premature rupture of membranes;Prolactin;Vaginal washing fluid Premature rupture of membranes;Prolactin;Vaginal washing fluid 541 548 http://www.ircmj.com/index.php?page=article&article_id=2147 N Kariman N Kariman Nursing Midwifery Faculty, Shahid Beheshti ]omgsiuigrmtycal Sciences, Iran +98(912)306 94 12, n_kariman@yahoo.com;n_kariman@sbmu.ac.ir; Nursing Midwifery Faculty, Shahid Beheshti ]omgsiuigrmtycal Sciences, Iran +98(912)306 94 12, n_kariman@yahoo.com;n_kariman@sbmu.ac.ir Nursing Midwifery Faculty, Shahid Beheshti ]omgsiuigrmtycal Sciences, Iran +98(912)306 94 12, n_kariman@yahoo.com;n_kariman@sbmu.ac.ir; Nursing Midwifery Faculty, Shahid Beheshti ]omgsiuigrmtycal Sciences, Iran +98(912)306 94 12, n_kariman@yahoo.com;n_kariman@sbmu.ac.ir M Hedayati M Hedayati Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti Medical University, Iran Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti Medical University, Iran
en Ultrastructural and morphometrical changes of mice ovaries following experimentally induced copper poisoning Ultrastructural and morphometrical changes of mice ovaries following experimentally induced copper poisoning research-article research-article Background

Copper (Cu) is an essential trace element involved in normal reproduction but its overexposure may produce some detrimental effects. The aim of this study was to investigate the effects of copper sulfate poisoning on morphometery of mice ovarian structures and probable intracellular changes.

Methods

Thirty mature female mice were randomly allocated to control and two treatment groups. In treatment groups, two different doses of copper sulfate including 100 mg/kg and 200 mg/kg in 0.2 cc were applied once a day for 35 consecutive days by gavage. Control animals received normal saline using the same volume and similar method. Animals from each experimental group were sacrificed 14 and 35 days after the beginning of drug administration and the left ovaries were removed for stereological evaluations by light microscopy and right ovaries were obtained for preparing electron microscopic sections.

Results

The morphometrical results showed that only the number of antral follicles was decreased by 100 mg/kg copper sulfate on day 14 compared to the control group (P=0.043). Hence, higher copper dose or longer consumption period significantly reduced different classes of follicles and corpora lutea. With 100 mg/kg copper sulfate some mild ultrastructural cell damages such as decrease of zona pellucida thickness, limited vacuolated areas and nuclear envelop dilation were seen on day 14. Higher or longer Cu administration produced more detrimental effects including more vacuolated areas, presence of secondary lysosomes, irregularity in cell shape and segmented nuclei with condensed and marginated chromatin and more enlarged and damaged mitochondria.

Conclusion

New evidences of early as well as late intracellular damages of copper has been presented by accurate stereological and ultrastructural methods. Antral follicles was the most susceptible cells with the lower and shorter copper consumption and long term or higher dose of copper affected the whole of ovarian structures.

Background

Copper (Cu) is an essential trace element involved in normal reproduction but its overexposure may produce some detrimental effects. The aim of this study was to investigate the effects of copper sulfate poisoning on morphometery of mice ovarian structures and probable intracellular changes.

Methods

Thirty mature female mice were randomly allocated to control and two treatment groups. In treatment groups, two different doses of copper sulfate including 100 mg/kg and 200 mg/kg in 0.2 cc were applied once a day for 35 consecutive days by gavage. Control animals received normal saline using the same volume and similar method. Animals from each experimental group were sacrificed 14 and 35 days after the beginning of drug administration and the left ovaries were removed for stereological evaluations by light microscopy and right ovaries were obtained for preparing electron microscopic sections.

Results

The morphometrical results showed that only the number of antral follicles was decreased by 100 mg/kg copper sulfate on day 14 compared to the control group (P=0.043). Hence, higher copper dose or longer consumption period significantly reduced different classes of follicles and corpora lutea. With 100 mg/kg copper sulfate some mild ultrastructural cell damages such as decrease of zona pellucida thickness, limited vacuolated areas and nuclear envelop dilation were seen on day 14. Higher or longer Cu administration produced more detrimental effects including more vacuolated areas, presence of secondary lysosomes, irregularity in cell shape and segmented nuclei with condensed and marginated chromatin and more enlarged and damaged mitochondria.

Conclusion

New evidences of early as well as late intracellular damages of copper has been presented by accurate stereological and ultrastructural methods. Antral follicles was the most susceptible cells with the lower and shorter copper consumption and long term or higher dose of copper affected the whole of ovarian structures.

Copper poisoning;Electron microscopy;Morphometry;Ovary;Mice Copper poisoning;Electron microscopy;Morphometry;Ovary;Mice 558 568 http://www.ircmj.com/index.php?page=article&article_id=3898 H Babaei H Babaei Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir L Roshangar L Roshangar Neuroscience Research Center, Tabriz University of Medical Sciences, IRAN Neuroscience Research Center, Tabriz University of Medical Sciences, IRAN E Sakhaee E Sakhaee Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir J Abshenas J Abshenas Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN, P. O. Box: 7616914111 +98 34 13 20 29 18, Babaei_H@ mail. uk.ac.ir R Kheirandish R Kheirandish Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN. R Dehghani R Dehghani Graduated Student of Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN Graduated Student of Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, IRAN
en Lidocaine Cyclodextrin complex Ophthalmic Drop, a New Topical Anesthetic Choice Lidocaine Cyclodextrin complex Ophthalmic Drop, a New Topical Anesthetic Choice research-article research-article Background

Topical anesthesia is a safe and cost-effective method considered as the first-choice in many procedures. Due to the physiological characteristics of eye, most of the local anesthetics cannot efficiently penetrate through the conjunctiva deep to tenon. The aim of this pilot study was to find a new form of lidocaine to give a sufficient level of anesthesia.

Methods:

Lidocaine Cyclodextrin complex ophthalmic drop was produced and its pharmacological properties were studied [tested] in standard temperature and pressure. 30 patients (18 males, 12 females) with the mean age of 30.68±8.02 years enrolled in this clinical trial. All the patients were fully informed and signed the ethics committee consent forms. The patients were given tetracaine drop as the anesthetic: 3 drops separated 2 minute apart 10 min before the intervention. If we achieved a sufficient level of anesthesia, the procedure was done after. If the patient could not tolerate the procedure, the method was changed to lidocaine drop (administered after wash-out period like the first drop).The last option was conventional injection method if the patient could not tolerate the procedure with the second method either.We used this type of anesthesia for conventional procedures such as forced duction test, symblepharon, pterygium, and disport injection into extra-ocular muscles. All the procedures were done by one surgeon in a university hospital. We used a 0 to 10 visual analogue scale for pain and two 0 to 4 patient and physician satisfaction scales designed for this study.

Results

The mean pain score was 7.53±0.90 in group 1 and 3.03±1.83 in group 2 (P=0.00). Patient and surgeon satisfaction in group 1 were 1.33±0.48 and 1.40±0.56 respectively; while 3.23±1.00 and 3.56±0.77 for group 2 (P=0.00). Tetracaine drop could not induce sufficient anesthesia for none of the patients. Cyclodextrin based lidocaine drop was successful except For two patients for whom we changed the anesthesia to Sub-conjunctival injection method.

Conclusion

Our newly manufactured cyclodextrin based lidocaine eye drop could successfully induce sufficient anesthesia for 28 of 30 patients. Further studies with larger sample sizes are now being designed to find more clinical evidence about this method.

Background

Topical anesthesia is a safe and cost-effective method considered as the first-choice in many procedures. Due to the physiological characteristics of eye, most of the local anesthetics cannot efficiently penetrate through the conjunctiva deep to tenon. The aim of this pilot study was to find a new form of lidocaine to give a sufficient level of anesthesia.

Methods:

Lidocaine Cyclodextrin complex ophthalmic drop was produced and its pharmacological properties were studied [tested] in standard temperature and pressure. 30 patients (18 males, 12 females) with the mean age of 30.68±8.02 years enrolled in this clinical trial. All the patients were fully informed and signed the ethics committee consent forms. The patients were given tetracaine drop as the anesthetic: 3 drops separated 2 minute apart 10 min before the intervention. If we achieved a sufficient level of anesthesia, the procedure was done after. If the patient could not tolerate the procedure, the method was changed to lidocaine drop (administered after wash-out period like the first drop).The last option was conventional injection method if the patient could not tolerate the procedure with the second method either.We used this type of anesthesia for conventional procedures such as forced duction test, symblepharon, pterygium, and disport injection into extra-ocular muscles. All the procedures were done by one surgeon in a university hospital. We used a 0 to 10 visual analogue scale for pain and two 0 to 4 patient and physician satisfaction scales designed for this study.

Results

The mean pain score was 7.53±0.90 in group 1 and 3.03±1.83 in group 2 (P=0.00). Patient and surgeon satisfaction in group 1 were 1.33±0.48 and 1.40±0.56 respectively; while 3.23±1.00 and 3.56±0.77 for group 2 (P=0.00). Tetracaine drop could not induce sufficient anesthesia for none of the patients. Cyclodextrin based lidocaine drop was successful except For two patients for whom we changed the anesthesia to Sub-conjunctival injection method.

Conclusion

Our newly manufactured cyclodextrin based lidocaine eye drop could successfully induce sufficient anesthesia for 28 of 30 patients. Further studies with larger sample sizes are now being designed to find more clinical evidence about this method.

Drug Delivery;Lidocaine;Cyclodextrin;Ophthalmicdrop;Symblepharon; Pterygium;Dysport Drug Delivery;Lidocaine;Cyclodextrin;Ophthalmicdrop;Symblepharon; Pterygium;Dysport 569 573 http://www.ircmj.com/index.php?page=article&article_id=4531 AA Sabermoghadam Ranjbar AA Sabermoghadam Ranjbar Ophthalmology research center, Mashhad University of Medical Sciences, Iran Ophthalmology research center, Mashhad University of Medical Sciences, Iran O Rajabi O Rajabi Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences,, Iran Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences,, Iran R Salari R Salari Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences,, Iran Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences,, Iran H Ashraf H Ashraf Ow~mqmdysscvmwmo~gz seuerohmas{ista~mvorwiyeoocevmsemrskigoouwr, Mashhad University of Medical Sciences, Iran 989153213658, ashrafh821@mums.ac.ir; Ow~mqmdysscvmwmo~gz seuerohmas{ista~mvorwiyeoocevmsemrskigoouwr, Mashhad University of Medical Sciences, Iran 989153213658, ashrafh821@mums.ac.ir Ow~mqmdysscvmwmo~gz seuerohmas{ista~mvorwiyeoocevmsemrskigoouwr, Mashhad University of Medical Sciences, Iran 989153213658, ashrafh821@mums.ac.ir; Ow~mqmdysscvmwmo~gz seuerohmas{ista~mvorwiyeoocevmsemrskigoouwr, Mashhad University of Medical Sciences, Iran 989153213658, ashrafh821@mums.ac.ir
en Cutis marmorata telangiectatica congenita in a preterm newborn – Case report and literature review Cutis marmorata telangiectatica congenita in a preterm newborn – Case report and literature review case-report case-report Background

Cutis marmorata telangiectatica congenita (CMTC) is a sporadic congenital skin vascular abnormality. Significant number of patients has other congenital anomalies.

Case Report

We report a case of a preterm male newborn with cutis marmorata pattern presented on the skin of the face, right side of front of the trunk, whole back, glutei and both legs. Besides, microretrognatia and asymmetric, bad-formed, low-set ears were notable. Ophthalmologic findings showed visual impairment and pale optic nerve papilla. Monitoring of child showed mental underdevelopment and motor retardation.

Conclusion

CMTC is a rare finding with good prognosis of skin malformations, with an obligation of dermatologist and paediatrician to investigate other associated congenital anomalies.

Background

Cutis marmorata telangiectatica congenita (CMTC) is a sporadic congenital skin vascular abnormality. Significant number of patients has other congenital anomalies.

Case Report

We report a case of a preterm male newborn with cutis marmorata pattern presented on the skin of the face, right side of front of the trunk, whole back, glutei and both legs. Besides, microretrognatia and asymmetric, bad-formed, low-set ears were notable. Ophthalmologic findings showed visual impairment and pale optic nerve papilla. Monitoring of child showed mental underdevelopment and motor retardation.

Conclusion

CMTC is a rare finding with good prognosis of skin malformations, with an obligation of dermatologist and paediatrician to investigate other associated congenital anomalies.

Cutis marmorata telangictatica congenital;Van Lohuizen syndrome Cutis marmorata telangictatica congenital;Van Lohuizen syndrome 578 583 http://www.ircmj.com/index.php?page=article&article_id=1876 A Matic A Matic Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs; Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs; Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs S Pricic S Pricic Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs M Matic M Matic Clinical centre of Vojvodina, Dermatovenereological clinic, Serbia Clinical centre of Vojvodina, Dermatovenereological clinic, Serbia G Velisavljev Filipovic G Velisavljev Filipovic Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs Neonatology department, Institute for child and youth health care of Vojvodina, Serbia 381216612172, mamatic@open.telekom.rs A Ristivojevic A Ristivojevic Neonatology department, Gynaecologic-obstetric clinic, Clinical centre of Vojvodina, Serbia Neonatology department, Gynaecologic-obstetric clinic, Clinical centre of Vojvodina, Serbia
en Decreased serum level of soluble-leptin-receptor in patients with systemic lupus erythematosus Decreased serum level of soluble-leptin-receptor in patients with systemic lupus erythematosus brief-report brief-report Background

There is some evidence suggesting that leptin and its negative regulator, soluble-leptinreceptor (SLR) may be able to influence inflammatory and autoimmune processes.

Methods

In this study, several variables including socio-demographics, health-related habits, depression score, serum molecules and blood parameters besides the SLR level were evaluated in patients with SLE (SLE-patients) and healthy controls.

Results

The patients had significantly lower SLR level and higher depression score than the controls and both of these variables have a significant association with the occurrence of disease in logistic regression model. Moreover, the results of Pearson correlation analysis showed that patients’ SLR level was negatively correlated with their weights and BDI scores.

Conclusion

For the first time, this study indicated a lower level of SLR in SLE-patients and suggested that lower concentrations of SLR in these patients may be implicated in the pathogenesis of SLE.

Background

There is some evidence suggesting that leptin and its negative regulator, soluble-leptinreceptor (SLR) may be able to influence inflammatory and autoimmune processes.

Methods

In this study, several variables including socio-demographics, health-related habits, depression score, serum molecules and blood parameters besides the SLR level were evaluated in patients with SLE (SLE-patients) and healthy controls.

Results

The patients had significantly lower SLR level and higher depression score than the controls and both of these variables have a significant association with the occurrence of disease in logistic regression model. Moreover, the results of Pearson correlation analysis showed that patients’ SLR level was negatively correlated with their weights and BDI scores.

Conclusion

For the first time, this study indicated a lower level of SLR in SLE-patients and suggested that lower concentrations of SLR in these patients may be implicated in the pathogenesis of SLE.

Soluble-leptin-receptor;Autoantibody;Systemic lupus erythematosus;Depression Soluble-leptin-receptor;Autoantibody;Systemic lupus erythematosus;Depression 587 593 http://www.ircmj.com/index.php?page=article&article_id=998 K Bagheri K Bagheri Department of Immunology, Faculty of medicine, Kazerun Branch, Islamic Azad University, P.O. Box: 73135 - 168,, Iran +989173118066;07118323702, Bagheri_kbz@yahoo.com;kbz.bagheri@gmail.com; Department of Immunology, Faculty of medicine, Kazerun Branch, Islamic Azad University, P.O. Box: 73135 - 168,, Iran +989173118066;07118323702, Bagheri_kbz@yahoo.com;kbz.bagheri@gmail.com Department of Immunology, Faculty of medicine, Kazerun Branch, Islamic Azad University, P.O. Box: 73135 - 168,, Iran +989173118066;07118323702, Bagheri_kbz@yahoo.com;kbz.bagheri@gmail.com; Department of Immunology, Faculty of medicine, Kazerun Branch, Islamic Azad University, P.O. Box: 73135 - 168,, Iran +989173118066;07118323702, Bagheri_kbz@yahoo.com;kbz.bagheri@gmail.com P Ebadi P Ebadi Department of Biochemistry, Faculty of medicine, Kazerun Branch, Islamic Azad University, Iran Department of Biochemistry, Faculty of medicine, Kazerun Branch, Islamic Azad University, Iran S Naeimi S Naeimi Department of Biochemistry, Faculty of medicine, Kazerun Branch, Islamic Azad University, Iran Department of Biochemistry, Faculty of medicine, Kazerun Branch, Islamic Azad University, Iran
en Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR brief-report brief-report Background

Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans.

Methods

We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7.

Result

PCR product of 336 bp was successfully amplified in a FLASH–PCR.

Conclusion

As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.

Background

Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans.

Methods

We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7.

Result

PCR product of 336 bp was successfully amplified in a FLASH–PCR.

Conclusion

As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.

E.coli O157:H7;Stx-1 gene;Detection;FLASH-PCR E.coli O157:H7;Stx-1 gene;Detection;FLASH-PCR 594 598 http://www.ircmj.com/index.php?page=article&article_id=1095 F Khatami F Khatami Department of Biology, Science School, Ferdowsi University, Iran. Department of Biology, Science School, Ferdowsi University, Iran. M Heidari M Heidari Department of Biology, Science School, Yazd University, Iran. +98 351 8122649, Heidarimm@yazduni.ac.ir; Department of Biology, Science School, Yazd University, Iran. +98 351 8122649, Heidarimm@yazduni.ac.ir Department of Biology, Science School, Yazd University, Iran. +98 351 8122649, Heidarimm@yazduni.ac.ir; Department of Biology, Science School, Yazd University, Iran. +98 351 8122649, Heidarimm@yazduni.ac.ir M Khatami M Khatami Department of Biology, Science School, Yazd University, Iran. +98 351 8122649, Heidarimm@yazduni.ac.ir Department of Biology, Science School, Yazd University, Iran. +98 351 8122649, Heidarimm@yazduni.ac.ir
en Double J-related hemoperitoneum in a living-related renal transplantation recipien Double J-related hemoperitoneum in a living-related renal transplantation recipien letter letter double-J;renal transplantation;hematoma;urinoma double-J;renal transplantation;hematoma;urinoma 599 601 http://www.ircmj.com/index.php?page=article&article_id=3885 Sh FTsai Sh FTsai Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com KH Shu KH Shu Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com; School of Medicine, Chung Shan Medical University, Taiwan Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com; School of Medicine, Chung Shan Medical University, Taiwan Cheng-Hsu Chen Cheng-Hsu Chen Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com; Department of Life Science, Tunghai University, Taiwan; School of Medicine, Chung Shan Medical University, Taiwan; Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com; Department of Life Science, Tunghai University, Taiwan; School of Medicine, Chung Shan Medical University, Taiwan; Division of Nephrology, Department of Motmsoom,mueicioo Ve}gzunw We~eranshow~mval Hospital, Taiwan 886-4-23592525;+98 912 183 7405, cschen920@yahoo.com